Intracellular Modulation, Extracellular Disposal and Serum Increase of MiR-150 Mark Lymphocyte Activation

Paola de Candia, Anna Torri, Tatiana Gorletta, Maya Fedeli, Elisabetta Bulgheroni, Cristina Cheroni, Francesco Marabita, Mariacristina Crosti, Monica Moro, Elena Pariani, Luisa Romanò, Susanna Esposito, Fabio Mosca, Grazisa Rossetti, Riccardo L. Rossi, Jens Geginat, Giulia Casorati, Paolo Dellabona, Massimiliano Pagani, Sergio Abrignani

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Activated lymphocytes release nano-sized vesicles (exosomes) containing microRNAs that can be monitored in the bloodstream. We asked whether elicitation of immune responses is followed by release of lymphocyte-specific microRNAs. We found that, upon activation in vitro, human and mouse lymphocytes down-modulate intracellular miR-150 and accumulate it in exosomes. In vivo, miR-150 levels increased significantly in serum of humans immunized with flu vaccines and in mice immunized with ovalbumin, and this increase correlated with elevation of antibody titers. Immunization of immune-deficient mice, lacking MHCII, resulted neither in antibody production nor in elevation of circulating miR-150. This study provides proof of concept that serum microRNAs can be detected, with minimally invasive procedure, as biomarkers of vaccination and more in general of adaptive immune responses. Furthermore, the prompt reduction of intracellular level of miR-150, a key regulator of mRNAs critical for lymphocyte differentiation and functions, linked to its release in the external milieu suggests that the selective extracellular disposal of microRNAs can be a rapid way to regulate gene expression during lymphocyte activation.

Original languageEnglish
Article numbere75348
JournalPLoS One
Volume8
Issue number9
DOIs
Publication statusPublished - Sep 26 2013

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Lymphocytes
lymphocyte proliferation
Lymphocyte Activation
MicroRNAs
microRNA
lymphocytes
Chemical activation
Modulation
exosomes
Exosomes
Serum
mice
Influenza Vaccines
ovalbumin
Ovalbumin
Immunization
Adaptive Immunity
antibody formation
influenza
blood flow

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

de Candia, P., Torri, A., Gorletta, T., Fedeli, M., Bulgheroni, E., Cheroni, C., ... Abrignani, S. (2013). Intracellular Modulation, Extracellular Disposal and Serum Increase of MiR-150 Mark Lymphocyte Activation. PLoS One, 8(9), [e75348]. https://doi.org/10.1371/journal.pone.0075348

Intracellular Modulation, Extracellular Disposal and Serum Increase of MiR-150 Mark Lymphocyte Activation. / de Candia, Paola; Torri, Anna; Gorletta, Tatiana; Fedeli, Maya; Bulgheroni, Elisabetta; Cheroni, Cristina; Marabita, Francesco; Crosti, Mariacristina; Moro, Monica; Pariani, Elena; Romanò, Luisa; Esposito, Susanna; Mosca, Fabio; Rossetti, Grazisa; Rossi, Riccardo L.; Geginat, Jens; Casorati, Giulia; Dellabona, Paolo; Pagani, Massimiliano; Abrignani, Sergio.

In: PLoS One, Vol. 8, No. 9, e75348, 26.09.2013.

Research output: Contribution to journalArticle

de Candia, P, Torri, A, Gorletta, T, Fedeli, M, Bulgheroni, E, Cheroni, C, Marabita, F, Crosti, M, Moro, M, Pariani, E, Romanò, L, Esposito, S, Mosca, F, Rossetti, G, Rossi, RL, Geginat, J, Casorati, G, Dellabona, P, Pagani, M & Abrignani, S 2013, 'Intracellular Modulation, Extracellular Disposal and Serum Increase of MiR-150 Mark Lymphocyte Activation', PLoS One, vol. 8, no. 9, e75348. https://doi.org/10.1371/journal.pone.0075348
de Candia, Paola ; Torri, Anna ; Gorletta, Tatiana ; Fedeli, Maya ; Bulgheroni, Elisabetta ; Cheroni, Cristina ; Marabita, Francesco ; Crosti, Mariacristina ; Moro, Monica ; Pariani, Elena ; Romanò, Luisa ; Esposito, Susanna ; Mosca, Fabio ; Rossetti, Grazisa ; Rossi, Riccardo L. ; Geginat, Jens ; Casorati, Giulia ; Dellabona, Paolo ; Pagani, Massimiliano ; Abrignani, Sergio. / Intracellular Modulation, Extracellular Disposal and Serum Increase of MiR-150 Mark Lymphocyte Activation. In: PLoS One. 2013 ; Vol. 8, No. 9.
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