Intracellular nucleotides of lymphocytes and granulocytes from normal ageing subjects

Rosa Maria Borzì, Paola Dal Monte, Mariagrazia Uguccioni, Riccardo Meliconi, Andrea Facchini

Research output: Contribution to journalArticle

Abstract

Impaired lymphocyte and granulocyte function in the aged may, in part, reflect intrinsic aged-related biochemical alterations. In this study we compared the ribonucleotide contents of lymphocytes and granulocytes from young and old subjects evaluated by means of an HPLC-anion exchange method. We found that in general both populations from old subjects present higher levels of the various nucleotides, in particular: ATP, UDP, CTP, UDP-glucose in granulocytes, AMP, CTP, UDP-N-acetylglucosamine, UDP-glucose in lymphocytes. These data suggest that these molecules accumulate in aged subjects because of altered biochemical pathways. The increased pool of UDP-sugars, in particular, could be due to a depressed activity of some glycosyltransferases which therefore fail to glycosylate some plasma membrane cell proteins, thus accounting for their functional impairment.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalMechanisms of Ageing and Development
Volume64
Issue number1-2
DOIs
Publication statusPublished - 1992

Keywords

  • Ageing
  • Granulocytes
  • HPLC
  • Lymphocytes
  • Nucleotides

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

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