TY - JOUR
T1 - Intracellular nucleotides of lymphocytes and granulocytes from normal ageing subjects
AU - Borzì, Rosa Maria
AU - Dal Monte, Paola
AU - Uguccioni, Mariagrazia
AU - Meliconi, Riccardo
AU - Facchini, Andrea
PY - 1992
Y1 - 1992
N2 - Impaired lymphocyte and granulocyte function in the aged may, in part, reflect intrinsic aged-related biochemical alterations. In this study we compared the ribonucleotide contents of lymphocytes and granulocytes from young and old subjects evaluated by means of an HPLC-anion exchange method. We found that in general both populations from old subjects present higher levels of the various nucleotides, in particular: ATP, UDP, CTP, UDP-glucose in granulocytes, AMP, CTP, UDP-N-acetylglucosamine, UDP-glucose in lymphocytes. These data suggest that these molecules accumulate in aged subjects because of altered biochemical pathways. The increased pool of UDP-sugars, in particular, could be due to a depressed activity of some glycosyltransferases which therefore fail to glycosylate some plasma membrane cell proteins, thus accounting for their functional impairment.
AB - Impaired lymphocyte and granulocyte function in the aged may, in part, reflect intrinsic aged-related biochemical alterations. In this study we compared the ribonucleotide contents of lymphocytes and granulocytes from young and old subjects evaluated by means of an HPLC-anion exchange method. We found that in general both populations from old subjects present higher levels of the various nucleotides, in particular: ATP, UDP, CTP, UDP-glucose in granulocytes, AMP, CTP, UDP-N-acetylglucosamine, UDP-glucose in lymphocytes. These data suggest that these molecules accumulate in aged subjects because of altered biochemical pathways. The increased pool of UDP-sugars, in particular, could be due to a depressed activity of some glycosyltransferases which therefore fail to glycosylate some plasma membrane cell proteins, thus accounting for their functional impairment.
KW - Ageing
KW - Granulocytes
KW - HPLC
KW - Lymphocytes
KW - Nucleotides
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U2 - 10.1016/0047-6374(92)90091-Q
DO - 10.1016/0047-6374(92)90091-Q
M3 - Article
C2 - 1630150
AN - SCOPUS:0026522376
VL - 64
SP - 1
EP - 11
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 1-2
ER -