Intracellular signaling pathways required for rat vascular smooth muscle cell migration: Interactions between basic fibroblast growth factor and platelet-derived growth factor

Claudio Bilato, Rebecca R. Pauly, Guido Melillo, Robert Monticone, Dan Gorelick-Feldman, Yehezkiel A. Gluzband, Steven J. Sollott, Bruce Ziman, Edward G. Lakatta, Michael T. Crow

Research output: Contribution to journalArticlepeer-review

Abstract

Intracellular signaling pathways activated by both PDGF and basic fibroblast growth factor (bFGF) have been implicated in the migration of vascular smooth muscle cells (VSMC), a key step in the pathogenesis of many vascular diseases. We demonstrate here that, while bFGF is a weak chemoattractant for VSMCs, it is required for the PDGF-directed migration of VSMCs and the activation of calcium/ calmodulin-dependent protein kinase II (CamKinase II), an intracellular event that we have previously shown to be important in the regulation of VSMC migration. Neutralizing antibodies to bFGF caused a dramatic reduction in the size of the intracellular calcium transient normally seen after PDGF stimulation and inhibited both PDGF-directed VSMC migration and CamKinase II activation. Partially restoring the calcium transient with ionomycin restored migration and CamKinase II activation as did the forced expression of a mutant CamKinase II that had been "locked" in the active state by site-directed mutagenesis. These results suggest that bFGF links PDGF receptor stimulation to changes in intracellular calcium and CamKinase II activation, reinforcing the central role played by CamKinase II in regulating VSMC migration.

Original languageEnglish
Pages (from-to)1905-1915
Number of pages11
JournalJournal of Clinical Investigation
Volume96
Issue number4
Publication statusPublished - Oct 1995

Keywords

  • Basic fibroblast growth factor
  • Calcium/calmodulin-dependent kinase II
  • Cell migration
  • Platelet-derived growth factor
  • Smooth muscle cells

ASJC Scopus subject areas

  • Medicine(all)

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