Intragenic amplification and formation of extrachromosomal small circular DNA molecules from the PIP gene on chromosome 7 in primary breast carcinomas

Monica Autiero, Alessandra Camarca, Marina Ciullo, Marie Anne Debily, Sandrine El Marhomy, Rosa Pasquinelli, Immacolata Capasso, Giuseppe D'Aiuto, Anna Maria Anzisi, Dominique Piatier-Tonneau, John Guardiola

Research output: Contribution to journalArticle

Abstract

The PIP gene is expressed in exocrine glands and, in pathologic conditions, in breast cysts and breast cancers exhibiting apocrine features. It is localized on the long arm of chromosome 7, a region frequently alterated in mammary tumors. We previously described an abnormal restriction pattern of the PIP gene in 33% of prostate carcinomas analyzed. Here, we analyze the structure of the PIP gene in primary breast carcinomas. We report that part of the 3′ end, including exon 3, intron C, two-thirds of exon 4 and a small portion of intron B, is amplified and involved in the formation of extrachromosomal spcDNA molecules in 3/14 (21.4%) breast cancers analyzed. The involvement of a well-defined intragenic region of a gene in the formation of spcDNA appears to be unprecedented. Since spcDNA has been suggested to serve as an enhancer of genetic instability, the PIP gene may be the target of genomic variability processes in breast cancer.

Original languageEnglish
Pages (from-to)370-377
Number of pages8
JournalInternational Journal of Cancer
Volume99
Issue number3
DOIs
Publication statusPublished - May 20 2002

Fingerprint

Circular DNA
Chromosomes, Human, Pair 7
Breast Neoplasms
Genes
Introns
Exons
Breast Cyst
Exocrine Glands
Prostate
Carcinoma

Keywords

  • Breast cancer
  • Chromosome 7
  • Genomic instability
  • PIP gene
  • SpcDNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Intragenic amplification and formation of extrachromosomal small circular DNA molecules from the PIP gene on chromosome 7 in primary breast carcinomas. / Autiero, Monica; Camarca, Alessandra; Ciullo, Marina; Debily, Marie Anne; El Marhomy, Sandrine; Pasquinelli, Rosa; Capasso, Immacolata; D'Aiuto, Giuseppe; Anzisi, Anna Maria; Piatier-Tonneau, Dominique; Guardiola, John.

In: International Journal of Cancer, Vol. 99, No. 3, 20.05.2002, p. 370-377.

Research output: Contribution to journalArticle

Autiero, M, Camarca, A, Ciullo, M, Debily, MA, El Marhomy, S, Pasquinelli, R, Capasso, I, D'Aiuto, G, Anzisi, AM, Piatier-Tonneau, D & Guardiola, J 2002, 'Intragenic amplification and formation of extrachromosomal small circular DNA molecules from the PIP gene on chromosome 7 in primary breast carcinomas', International Journal of Cancer, vol. 99, no. 3, pp. 370-377. https://doi.org/10.1002/ijc.10368
Autiero, Monica ; Camarca, Alessandra ; Ciullo, Marina ; Debily, Marie Anne ; El Marhomy, Sandrine ; Pasquinelli, Rosa ; Capasso, Immacolata ; D'Aiuto, Giuseppe ; Anzisi, Anna Maria ; Piatier-Tonneau, Dominique ; Guardiola, John. / Intragenic amplification and formation of extrachromosomal small circular DNA molecules from the PIP gene on chromosome 7 in primary breast carcinomas. In: International Journal of Cancer. 2002 ; Vol. 99, No. 3. pp. 370-377.
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AB - The PIP gene is expressed in exocrine glands and, in pathologic conditions, in breast cysts and breast cancers exhibiting apocrine features. It is localized on the long arm of chromosome 7, a region frequently alterated in mammary tumors. We previously described an abnormal restriction pattern of the PIP gene in 33% of prostate carcinomas analyzed. Here, we analyze the structure of the PIP gene in primary breast carcinomas. We report that part of the 3′ end, including exon 3, intron C, two-thirds of exon 4 and a small portion of intron B, is amplified and involved in the formation of extrachromosomal spcDNA molecules in 3/14 (21.4%) breast cancers analyzed. The involvement of a well-defined intragenic region of a gene in the formation of spcDNA appears to be unprecedented. Since spcDNA has been suggested to serve as an enhancer of genetic instability, the PIP gene may be the target of genomic variability processes in breast cancer.

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