Intraglomerular and interstitial leukocyte infiltration, adhesion molecules, and interleukin-1α expression in 15 cases of antineutrophil cytoplasmic autoantibody-associated renal vasculitis

M. P. Rastaldi, F. Ferrario, S. Tunesi, L. Yang, G. D'Amico

Research output: Contribution to journalArticle

Abstract

In renal biopsy specimens from 15 patients with antineutrophil cytoplasmic autoantibody (ANCA)-associated renal vasculitis, the infiltrating intraglomerular and interstitial leukocytes were localized and the adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and the cytokine interleukin-1α (IL-1α) were studied by an immunohistochemical method. Intraglomerular leukocytes were mainly macrophages (13.46 ± 9.29 cells/glomerular cross-section) and, to a lesser extent, T lymphocytes (4.61 ± 2.81 cells/glomerular cross-section). Staining with VCAM-1, which was negative in the undamaged tufts, was strongly positive in necrotizing-extracapillary lesions. Staining with ICAM-1 was also present in the damaged tufts, but its pattern was more diffuse. Intraglomerular IL-1α was found in all biopsy specimens. Where the Bowman's capsule was not damaged, the periglomerular infiltrating cells wars macrophages (42.6 ± 25.2 cells/glomerular cross-section) and T lymphocytes (51.06 ± 33.0 cells/glomerular cross-section). When there was a granulomatous lesion involving the glomerulus, the number of cells per granulomatous area revealed a massive number of CD45-positive leukocytes (345.83 ± 237.47 cells/granulomatous lesion), many of them positive for activity markers (HLA-DR, IL-2R), adhesion molecules, and IL-1α. Many activated cells were also present in interstitial areas of perivascular clusters of leukocytes, in which T lymphocytes (prevalently CD4+ cells) outnumbered the macrophages (331.55 ± 207.85 cells/0.05 mm2 area v 125.68 ± 60.57 cells/0.05 mm2 area). Adhesion molecules and IL-1α were found in both tubular and vascular areas in all biopsy specimens. Our data strongly support the involvement of both the adhesion molecules ICAM-1 and VCAM-1 in the recruitment of intraglomerular leukocytes in renal vasculitis, indicate that VCAM-1 is a very good marker of necrotizing-extracapillary damage, and suggest its crucial connection with the macrophage recruitment in these vasculitic lesions. The presence of histochemically detectable levels of IL- 1α in glomeruli, tubules, and vessels and on some inflammatory cells supports its involvement in the vasculitic lesions, probably by triggering a positive feedback that increases the damage.

Original languageEnglish
Pages (from-to)48-57
Number of pages10
JournalAmerican Journal of Kidney Diseases
Volume27
Issue number1
Publication statusPublished - 1996

Keywords

  • adhesion molecules
  • antineutrophil cytoplasmic autoantibody-associated renal vasculitis
  • intercellular adhesion molecule-1
  • interleukin- 1α
  • Leukocyte infiltration
  • necrotizing/extracapillary damage
  • vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Nephrology

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