Intramyocellular triglyceride content is a determinant of in vivo insulin resistance in humans: A 1H-13C nuclear magnetic resonance spectroscopy assessment in offspring of type 2 diabetic parents

Gianluca Perseghin, Paola Scifo, Francesco De Cobelli, Emanuela Pagliato, Alberto Battezzati, Cinzia Arcelloni, Angelo Vanzulli, Giulio Testolin, Guido Pozza, Alessandro Del Maschio, Livio Luzi

Research output: Contribution to journalArticle

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Abstract

Insulin resistance is the best prediction factor for the clinical onset of type 2 diabetes. It was suggested that intramuscular triglyceride store may be a primary pathogenic factor for its development. To test this hypothesis, 14 young lean offspring of type 2 diabetic parents, a model of in vivo insulin resistance with increased risk to develop diabetes, and 14 healthy subjects matched for anthropomorphic parameters and life habits were studied with 1) euglycemic-hyperinsulinemic clamp to assess whole body insulin sensitivity, 2) localized 1H nuclear magnetic resonance (NMR) spectroscopy of the soleus (higher content of fiber type I, insulin sensitive) and tibialis anterior (higher content of fiber type IIb, less insulin sensitive) muscles to assess intramyocellular triglyceride content, 3) NMR of the calf subcutaneous adipose tissue to assess composition in saturated/unsaturated carbons of triglyceride fatty acid chains, and 4) dual X-ray energy absorption to assess body composition. Offspring of diabetic parents, notwithstanding normal fat content and distribution, were characterized by insulin resistance and increased intramyocellular triglyceride content in the soleus (P <0.01) but not in the tibialis anterior (P = 0.19), but showed a normal content of saturated/unsaturated carbons in the fatty acid chain of subcutaneous adipocytes. Stepwise regression analysis selected intramyocellular triglyceride soleus content and plasma free fatty acid levels as the main predictors of whole body insulin sensitivity. In conclusion, 1H and 13C NMR spectroscopy revealed intramyocellular abnormalities of lipid metabolism associated with whole body insulin resistance in subjects at high risk of developing diabetes, and might be useful tools for noninvasively monitoring these alterations in diabetes and prediabetic states.

Original languageEnglish
Pages (from-to)1600-1606
Number of pages7
JournalDiabetes
Volume48
Issue number8
DOIs
Publication statusPublished - Aug 1999

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Insulin Resistance
Triglycerides
Magnetic Resonance Spectroscopy
Parents
Fatty Acids
Carbon
Insulin
Prediabetic State
Glucose Clamp Technique
Subcutaneous Fat
Body Composition
Lipid Metabolism
Nonesterified Fatty Acids
Adipocytes
Type 2 Diabetes Mellitus
Habits
Healthy Volunteers
Fats
Regression Analysis
X-Rays

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Intramyocellular triglyceride content is a determinant of in vivo insulin resistance in humans : A 1H-13C nuclear magnetic resonance spectroscopy assessment in offspring of type 2 diabetic parents. / Perseghin, Gianluca; Scifo, Paola; De Cobelli, Francesco; Pagliato, Emanuela; Battezzati, Alberto; Arcelloni, Cinzia; Vanzulli, Angelo; Testolin, Giulio; Pozza, Guido; Del Maschio, Alessandro; Luzi, Livio.

In: Diabetes, Vol. 48, No. 8, 08.1999, p. 1600-1606.

Research output: Contribution to journalArticle

Perseghin, Gianluca ; Scifo, Paola ; De Cobelli, Francesco ; Pagliato, Emanuela ; Battezzati, Alberto ; Arcelloni, Cinzia ; Vanzulli, Angelo ; Testolin, Giulio ; Pozza, Guido ; Del Maschio, Alessandro ; Luzi, Livio. / Intramyocellular triglyceride content is a determinant of in vivo insulin resistance in humans : A 1H-13C nuclear magnetic resonance spectroscopy assessment in offspring of type 2 diabetic parents. In: Diabetes. 1999 ; Vol. 48, No. 8. pp. 1600-1606.
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AU - Battezzati, Alberto

AU - Arcelloni, Cinzia

AU - Vanzulli, Angelo

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AU - Del Maschio, Alessandro

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