Intranasal delivery of DNA encoding antigens of Mycobacterium tuberculosis by non-pathogenic invasive Escherichia coli

Paola Brun, Antonella Zumbo, Ignazio Castagliuolo, Giovanni Delogu, Filippo Manfrin, Michela Sali, Giovanni Fadda, Catherine Grillot-Courvalin, Giorgio Palù, Riccardo Manganelli

Research output: Contribution to journalArticle

Abstract

Naturally invasive bacteria have been successfully used for mucosal delivery of DNA vaccines against bacterial, viral and tumour antigens. Recently, an alternative delivery system based on a genetically modified mutant of the non-pathogenic commensal bacterium Escherichia coli, was developed and successfully used to deliver therapeutic genes and immunogenic proteins to epithelial cells in vivo. In this work, we used these recombinant invasive bacteria to deliver DNA vaccines against two Mycobacterium tuberculosis proteins (FbpA, and HtpX) following intranasal administration. Both DNA vaccines were able to induce an antigen-specific T-cell response. Moreover, mice immunized with the recombinant bacteria carrying the DNA vaccine encoding HtpX, were significatively protected from challenge with M. tuberculosis.

Original languageEnglish
Pages (from-to)1934-1941
Number of pages8
JournalVaccine
Volume26
Issue number16
DOIs
Publication statusPublished - Apr 7 2008

Keywords

  • Delivery system
  • DNA vaccine
  • Live recombinant vaccine
  • Mycobacterium tuberculosis
  • Tuberculosis

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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