Intraperitoneal radio-localization of tumors pre-targeted by biotinylated monoclonal antibodies

G. Paganelli, S. Pervez, A. G. Siccardi, G. Rowlinson, G. Deleide, F. Chiolerio, M. Malcovati, G. A. Scassellati, A. A. Epenetos

Research output: Contribution to journalArticlepeer-review

Abstract

We describe 2-step and 3-step strategies for intraperitoneal tumor radio-localization by means of monoclonal antibodies (MAbs). Nude mice bearing intraperitoneal human colon carcinoma tumors were injected i.p. with biotinylated MAb AUA1, followed 24 hr later by radioiodinated streptavidin (2-ste). The uptake of radioactivity in tumor and normal tissues was measured 4 hr after injection of radioactive compound. A 3-step strategy consisted in administering biotinylated antibody, cold avidin after 24 hr and 111In-labelled biotin after a further 4 hr; mice were then killed 2 hr later. Tumor localization of intraperitoneally-administered biotinylated antibody and direct targeting of radioactive streptavidin to biotinylated antibody bound to tumor sites were demonstrated using immunohistochemistry and autoradiography. Our results show that (i) the 2-step approach increased the percentage of radioactivity uptake by tumor with respect to directly labelled antibodies (24% vs 6%) and improved the tumor/non-tumor ratio; (ii) the 3-step approach allowed faster blood clearance of the radioactive probe (111In-biotin) and yielded high tumor/non-tumour ratios. 'Pre-targeting' methods appear to have advantages over the conventional 1-step approach with directly radiolabelled antibody.

Original languageEnglish
Pages (from-to)1184-1189
Number of pages6
JournalInternational Journal of Cancer
Volume45
Issue number6
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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