Intraspecies transmission of BASE induces clinical dullness and amyotrophic changes

Guerino Lombardi, Cristina Casalone, Antonio D'Angelo, Daniela Gelmetti, Gloria Torcoli, Ilaria Barbieri, Cristiano Corona, Elisa Fasoli, Alessia Farinazzo, Michele Fiorini, Matteo Gelati, Barbara Iulini, Fabrizio Tagliavini, Sergio Ferrari, Maria Caramelli, Salvatore Monaco, Lorenzo Capucci, Gianluigi Zanusso

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Abstract

The disease phenotype of bovine spongiform encephalopathy (BSE) and the molecular/ biological properties of its prion strain, including the host range and the characteristics of BSE-related disorders, have been extensively studied since its discovery in 1986. In recent years, systematic testing of the brains of cattle coming to slaughter resulted in the identification of at least two atypical forms of BSE. These emerging disorders are characterized by novel conformers of the bovine pathological prion protein (PrPTSE), named high-type (BSE-H) and low-type (BSE-L). We recently reported two Italian atypical cases with a PrPTSE type identical to BSE-L, pathologically characterized by PrP amyloid plaques and known as bovine amyloidotic spongiform encephalopathy (BASE). Several lines of evidence suggest that BASE is highly virulent and easily transmissible to a wide host range. Experimental transmission to transgenic mice overexpressing bovine PrP (Tgbov XV) suggested that BASE is caused by a prion strain distinct from the BSE isolate. In the present study, we experimentally infected Friesian and Alpine brown cattle with Italian BSE and BASE isolates via the intracerebral route. BASE-infected cattle developed amyotrophic changes accompanied by mental dullness. The molecular and neuropathological profiles, including PrP deposition pattern, closely matched those observed in the original cases. This study provides clear evidence of BASE as a distinct prion isolate and discloses a novel disease phenotype in cattle.

Original languageEnglish
Article numbere1000075
JournalPLoS Pathogens
Volume4
Issue number5
DOIs
Publication statusPublished - May 2008

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

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    Lombardi, G., Casalone, C., D'Angelo, A., Gelmetti, D., Torcoli, G., Barbieri, I., Corona, C., Fasoli, E., Farinazzo, A., Fiorini, M., Gelati, M., Iulini, B., Tagliavini, F., Ferrari, S., Caramelli, M., Monaco, S., Capucci, L., & Zanusso, G. (2008). Intraspecies transmission of BASE induces clinical dullness and amyotrophic changes. PLoS Pathogens, 4(5), [e1000075]. https://doi.org/10.1371/journal.ppat.1000075