Supplementazione e.v. di acido folinico e vitamina B12 e concentrazioni di omocisteina nei pazienti emodializzati.

Translated title of the contribution: Intravenous folinic acid and vitamin B12 supplementation and homocysteine concentration in hemodialysis patients

E. Corghi, C. Patrosso, F. Bamonti, I. Baragetti, C. Novembrino, G. Lando, M. De Franceschi, G. Buccianti

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

INTRODUCTION: Hyperhomocysteinemia is one of the causes of the increased incidence of cardiovascular disease in uremia. Since homocysteine (Hcy) metabolism depends on the availability of folate and vitamin B12, we have measured the effects of chronic i.v. supplementation of folinic acid and vitamin B12 in a group of patients on maintenance hemodialysis. METHODS: We compared the blood concentration of total Hcy (tHcy), vitamin B12 and folate and the intraerythrocyte concentration of folate in a group of 27 hemodialysis patients (Treated group), given an i.v supplementation with folinic acid (0.9 mg) and Vitamin B12 (cyanocobalamine 1.5 mg and hydroxycobalamine 1.5 mg) three times per week at the end of each dialysis session with those measured in a similar group of 28 hemodialysis patients without supplementation (No Treatment group). The patients were also characterized for the thermolabile variant (mutation C667-->T) of the enzyme methylene-tetrahydrofolate reductase (tMTHFR). RESULTS: High plasma levels (<11.7 micromol/L) of tHcy were observed in 54/55 patients. T patients had Hcy values significantly lower than NT ones (31.7+/-3.6 vs. 1.1+/-8.3micromol/L, p <0.05). Serum vitamin B12 (1200 73.6 vs. 762+/-72.2 pmol/L, p <0.001) and intraerythrocyte folate levels were also significantly higher in the T group (2176+/-127 vs. 1511+/-156, p <0.005), while no significant difference was observed for serum folate. The distribution of tMTHFR genotypes was similar in the two groups. Homozygous patients showed higher levels of Hcy in comparison with wild type patients both in the whole population (62.32+/-15.9 vs.30.43+/-3.2, p <0.05) and in the NT group (87.8+/-25.3 vs.36.8+/-13.1., p <0.05), while no significant difference was observed among genotypes in the T group. CONCLUSIONS: Uremic patients on hemodialysis, when supplemented with even low i.v. dose of folinic acid and vitamin B12, show significantly lower plasma levels of tHcy than non-supplemented patients.

Original languageItalian
Pages (from-to)301-307
Number of pages7
JournalGiornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia
Volume19
Issue number3
Publication statusPublished - May 2002

Fingerprint

Leucovorin
Homocysteine
Vitamin B 12
Renal Dialysis
Folic Acid
Methylenetetrahydrofolate Reductase (NADPH2)
Genotype
Hyperhomocysteinemia
Mutation
Uremia
Enzymes
Serum
Dialysis
Cardiovascular Diseases
Maintenance

ASJC Scopus subject areas

  • Nephrology

Cite this

Supplementazione e.v. di acido folinico e vitamina B12 e concentrazioni di omocisteina nei pazienti emodializzati. / Corghi, E.; Patrosso, C.; Bamonti, F.; Baragetti, I.; Novembrino, C.; Lando, G.; De Franceschi, M.; Buccianti, G.

In: Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia, Vol. 19, No. 3, 05.2002, p. 301-307.

Research output: Contribution to journalArticle

Corghi, E. ; Patrosso, C. ; Bamonti, F. ; Baragetti, I. ; Novembrino, C. ; Lando, G. ; De Franceschi, M. ; Buccianti, G. / Supplementazione e.v. di acido folinico e vitamina B12 e concentrazioni di omocisteina nei pazienti emodializzati. In: Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia. 2002 ; Vol. 19, No. 3. pp. 301-307.
@article{70038711db5b4667a61b1c2d20d818c5,
title = "Supplementazione e.v. di acido folinico e vitamina B12 e concentrazioni di omocisteina nei pazienti emodializzati.",
abstract = "INTRODUCTION: Hyperhomocysteinemia is one of the causes of the increased incidence of cardiovascular disease in uremia. Since homocysteine (Hcy) metabolism depends on the availability of folate and vitamin B12, we have measured the effects of chronic i.v. supplementation of folinic acid and vitamin B12 in a group of patients on maintenance hemodialysis. METHODS: We compared the blood concentration of total Hcy (tHcy), vitamin B12 and folate and the intraerythrocyte concentration of folate in a group of 27 hemodialysis patients (Treated group), given an i.v supplementation with folinic acid (0.9 mg) and Vitamin B12 (cyanocobalamine 1.5 mg and hydroxycobalamine 1.5 mg) three times per week at the end of each dialysis session with those measured in a similar group of 28 hemodialysis patients without supplementation (No Treatment group). The patients were also characterized for the thermolabile variant (mutation C667-->T) of the enzyme methylene-tetrahydrofolate reductase (tMTHFR). RESULTS: High plasma levels (<11.7 micromol/L) of tHcy were observed in 54/55 patients. T patients had Hcy values significantly lower than NT ones (31.7+/-3.6 vs. 1.1+/-8.3micromol/L, p <0.05). Serum vitamin B12 (1200 73.6 vs. 762+/-72.2 pmol/L, p <0.001) and intraerythrocyte folate levels were also significantly higher in the T group (2176+/-127 vs. 1511+/-156, p <0.005), while no significant difference was observed for serum folate. The distribution of tMTHFR genotypes was similar in the two groups. Homozygous patients showed higher levels of Hcy in comparison with wild type patients both in the whole population (62.32+/-15.9 vs.30.43+/-3.2, p <0.05) and in the NT group (87.8+/-25.3 vs.36.8+/-13.1., p <0.05), while no significant difference was observed among genotypes in the T group. CONCLUSIONS: Uremic patients on hemodialysis, when supplemented with even low i.v. dose of folinic acid and vitamin B12, show significantly lower plasma levels of tHcy than non-supplemented patients.",
author = "E. Corghi and C. Patrosso and F. Bamonti and I. Baragetti and C. Novembrino and G. Lando and {De Franceschi}, M. and G. Buccianti",
year = "2002",
month = "5",
language = "Italian",
volume = "19",
pages = "301--307",
journal = "Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia",
issn = "0393-5590",
publisher = "Wichtig Publishing Srl",
number = "3",

}

TY - JOUR

T1 - Supplementazione e.v. di acido folinico e vitamina B12 e concentrazioni di omocisteina nei pazienti emodializzati.

AU - Corghi, E.

AU - Patrosso, C.

AU - Bamonti, F.

AU - Baragetti, I.

AU - Novembrino, C.

AU - Lando, G.

AU - De Franceschi, M.

AU - Buccianti, G.

PY - 2002/5

Y1 - 2002/5

N2 - INTRODUCTION: Hyperhomocysteinemia is one of the causes of the increased incidence of cardiovascular disease in uremia. Since homocysteine (Hcy) metabolism depends on the availability of folate and vitamin B12, we have measured the effects of chronic i.v. supplementation of folinic acid and vitamin B12 in a group of patients on maintenance hemodialysis. METHODS: We compared the blood concentration of total Hcy (tHcy), vitamin B12 and folate and the intraerythrocyte concentration of folate in a group of 27 hemodialysis patients (Treated group), given an i.v supplementation with folinic acid (0.9 mg) and Vitamin B12 (cyanocobalamine 1.5 mg and hydroxycobalamine 1.5 mg) three times per week at the end of each dialysis session with those measured in a similar group of 28 hemodialysis patients without supplementation (No Treatment group). The patients were also characterized for the thermolabile variant (mutation C667-->T) of the enzyme methylene-tetrahydrofolate reductase (tMTHFR). RESULTS: High plasma levels (<11.7 micromol/L) of tHcy were observed in 54/55 patients. T patients had Hcy values significantly lower than NT ones (31.7+/-3.6 vs. 1.1+/-8.3micromol/L, p <0.05). Serum vitamin B12 (1200 73.6 vs. 762+/-72.2 pmol/L, p <0.001) and intraerythrocyte folate levels were also significantly higher in the T group (2176+/-127 vs. 1511+/-156, p <0.005), while no significant difference was observed for serum folate. The distribution of tMTHFR genotypes was similar in the two groups. Homozygous patients showed higher levels of Hcy in comparison with wild type patients both in the whole population (62.32+/-15.9 vs.30.43+/-3.2, p <0.05) and in the NT group (87.8+/-25.3 vs.36.8+/-13.1., p <0.05), while no significant difference was observed among genotypes in the T group. CONCLUSIONS: Uremic patients on hemodialysis, when supplemented with even low i.v. dose of folinic acid and vitamin B12, show significantly lower plasma levels of tHcy than non-supplemented patients.

AB - INTRODUCTION: Hyperhomocysteinemia is one of the causes of the increased incidence of cardiovascular disease in uremia. Since homocysteine (Hcy) metabolism depends on the availability of folate and vitamin B12, we have measured the effects of chronic i.v. supplementation of folinic acid and vitamin B12 in a group of patients on maintenance hemodialysis. METHODS: We compared the blood concentration of total Hcy (tHcy), vitamin B12 and folate and the intraerythrocyte concentration of folate in a group of 27 hemodialysis patients (Treated group), given an i.v supplementation with folinic acid (0.9 mg) and Vitamin B12 (cyanocobalamine 1.5 mg and hydroxycobalamine 1.5 mg) three times per week at the end of each dialysis session with those measured in a similar group of 28 hemodialysis patients without supplementation (No Treatment group). The patients were also characterized for the thermolabile variant (mutation C667-->T) of the enzyme methylene-tetrahydrofolate reductase (tMTHFR). RESULTS: High plasma levels (<11.7 micromol/L) of tHcy were observed in 54/55 patients. T patients had Hcy values significantly lower than NT ones (31.7+/-3.6 vs. 1.1+/-8.3micromol/L, p <0.05). Serum vitamin B12 (1200 73.6 vs. 762+/-72.2 pmol/L, p <0.001) and intraerythrocyte folate levels were also significantly higher in the T group (2176+/-127 vs. 1511+/-156, p <0.005), while no significant difference was observed for serum folate. The distribution of tMTHFR genotypes was similar in the two groups. Homozygous patients showed higher levels of Hcy in comparison with wild type patients both in the whole population (62.32+/-15.9 vs.30.43+/-3.2, p <0.05) and in the NT group (87.8+/-25.3 vs.36.8+/-13.1., p <0.05), while no significant difference was observed among genotypes in the T group. CONCLUSIONS: Uremic patients on hemodialysis, when supplemented with even low i.v. dose of folinic acid and vitamin B12, show significantly lower plasma levels of tHcy than non-supplemented patients.

UR - http://www.scopus.com/inward/record.url?scp=1842856708&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842856708&partnerID=8YFLogxK

M3 - Articolo

C2 - 12195398

AN - SCOPUS:1842856708

VL - 19

SP - 301

EP - 307

JO - Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia

JF - Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia

SN - 0393-5590

IS - 3

ER -