TY - JOUR
T1 - Intrinsic phenotypic diversity of embryonic and fetal myoblasts is revealed by genome-wide gene expression analysis on purified cells
AU - Biressi, Stefano
AU - Tagliafico, Enrico
AU - Lamorte, Giuseppe
AU - Monteverde, Stefania
AU - Tenedini, Elena
AU - Roncaglia, Enrica
AU - Ferrari, Sergio
AU - Ferrari, Stefano
AU - Cusella-De Angelis, Maria Gabriella
AU - Tajbakhsh, Shahragim
AU - Cossu, Giulio
PY - 2007/4/15
Y1 - 2007/4/15
N2 - Skeletal muscle development occurs asynchronously and it has been proposed to be dependent upon the generation of temporally distinct populations of myogenic cells. This long-held hypothesis has not been tested directly due to the inability to isolate and analyze purified populations of myoblasts derived from specific stages of prenatal development. Using a mouse strain with the GFP reporter gene targeted into the Myf5 locus, a cell-sorting method was developed for isolating embryonic and fetal myoblasts. The two types of myoblasts show an intrinsic difference in fusion ability, proliferation, differentiation and response to TGFβ, TPA and BMP-4 in vitro. Microarray and quantitative PCR were used to identify differentially expressed genes both before and after differentiation, thus allowing a precise phenotypic analysis of the two populations. Embryonic and fetal myoblasts differ in the expression of a number of transcription factors and surface molecules, which may control different developmental programs. For example, only embryonic myoblasts express a Hox code along the antero-posterior axis, indicating that they possess direct positional information. Taken together, the data presented here demonstrate that embryonic and fetal myoblasts represent intrinsically different myogenic lineages and provide important information for the understanding of the molecular mechanisms governing skeletal muscle development.
AB - Skeletal muscle development occurs asynchronously and it has been proposed to be dependent upon the generation of temporally distinct populations of myogenic cells. This long-held hypothesis has not been tested directly due to the inability to isolate and analyze purified populations of myoblasts derived from specific stages of prenatal development. Using a mouse strain with the GFP reporter gene targeted into the Myf5 locus, a cell-sorting method was developed for isolating embryonic and fetal myoblasts. The two types of myoblasts show an intrinsic difference in fusion ability, proliferation, differentiation and response to TGFβ, TPA and BMP-4 in vitro. Microarray and quantitative PCR were used to identify differentially expressed genes both before and after differentiation, thus allowing a precise phenotypic analysis of the two populations. Embryonic and fetal myoblasts differ in the expression of a number of transcription factors and surface molecules, which may control different developmental programs. For example, only embryonic myoblasts express a Hox code along the antero-posterior axis, indicating that they possess direct positional information. Taken together, the data presented here demonstrate that embryonic and fetal myoblasts represent intrinsically different myogenic lineages and provide important information for the understanding of the molecular mechanisms governing skeletal muscle development.
KW - Embryonic myoblast
KW - Fetal myoblast
KW - Fiber diversity
KW - Hox
KW - Microarray
KW - Notch
KW - TGFβ
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UR - http://www.scopus.com/inward/citedby.url?scp=34047261093&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2007.01.016
DO - 10.1016/j.ydbio.2007.01.016
M3 - Article
C2 - 17292343
AN - SCOPUS:34047261093
VL - 304
SP - 633
EP - 651
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -