Intrinsic phenotypic diversity of embryonic and fetal myoblasts is revealed by genome-wide gene expression analysis on purified cells

Stefano Biressi, Enrico Tagliafico, Giuseppe Lamorte, Stefania Monteverde, Elena Tenedini, Enrica Roncaglia, Sergio Ferrari, Stefano Ferrari, Maria Gabriella Cusella-De Angelis, Shahragim Tajbakhsh, Giulio Cossu

Research output: Contribution to journalArticlepeer-review

Abstract

Skeletal muscle development occurs asynchronously and it has been proposed to be dependent upon the generation of temporally distinct populations of myogenic cells. This long-held hypothesis has not been tested directly due to the inability to isolate and analyze purified populations of myoblasts derived from specific stages of prenatal development. Using a mouse strain with the GFP reporter gene targeted into the Myf5 locus, a cell-sorting method was developed for isolating embryonic and fetal myoblasts. The two types of myoblasts show an intrinsic difference in fusion ability, proliferation, differentiation and response to TGFβ, TPA and BMP-4 in vitro. Microarray and quantitative PCR were used to identify differentially expressed genes both before and after differentiation, thus allowing a precise phenotypic analysis of the two populations. Embryonic and fetal myoblasts differ in the expression of a number of transcription factors and surface molecules, which may control different developmental programs. For example, only embryonic myoblasts express a Hox code along the antero-posterior axis, indicating that they possess direct positional information. Taken together, the data presented here demonstrate that embryonic and fetal myoblasts represent intrinsically different myogenic lineages and provide important information for the understanding of the molecular mechanisms governing skeletal muscle development.

Original languageEnglish
Pages (from-to)633-651
Number of pages19
JournalDevelopmental Biology
Volume304
Issue number2
DOIs
Publication statusPublished - Apr 15 2007

Keywords

  • Embryonic myoblast
  • Fetal myoblast
  • Fiber diversity
  • Hox
  • Microarray
  • Notch
  • TGFβ

ASJC Scopus subject areas

  • Developmental Biology

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