Intrinsically disordered amphiphilic peptides as potential targets in drug delivery vehicles

Marian Vincenzi, Antonella Accardo, Susan Costantini, Stefania Scala, Luigi Portella, Annamaria Trotta, Luisa Ronga, Jean Guillon, Marilisa Leone, Giovanni Colonna, Filomena Rossi, Diego Tesauro

Research output: Contribution to journalArticle

Abstract

Intrinsically disordered proteins/peptides play a crucial role in many physiological and pathological events and may assume a precise conformation upon binding to a specific target. Recently, we have described the conformational and functional properties of two linear ester peptides provided with the following sequences: Y-G-E-C-P-C-K-OAllyl (PepK) and Y-G-E-C-P-C-E-OAllyl (PepE). Both peptides are characterized by the presence of the "CPC" motif together with a few amino acids able to promote disorder. The CPC sequence is a binding motif for the CXCR4 receptor that represents a well-known target for cancer therapies. In this paper, we report on synthetic amphiphilic peptides that consist of lipophilic derivatives of PepE and PepK bearing two stearic alkyl chains and/or an ethoxylic spacer. These peptide amphiphiles form stable supramolecular aggregates; they present conformational features that are typical of intrinsically disordered molecules as shown by CD spectroscopy. Solution fluorescence and DLS studies have been performed to evaluate Critical Micellar Concentrations and the dimension of supramolecular aggregates. Moreover, preliminary in vitro cell-based assays have been conducted to investigate the molecular recognition processes involving the CXCR4 receptor. In the end, the results obtained have been compared with the previous data generated by the corresponding non-amphiphilic peptides (PepE and PepK).

Original languageEnglish
Pages (from-to)2925-2932
Number of pages8
JournalMolecular BioSystems
Volume11
Issue number11
DOIs
Publication statusPublished - 2015

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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    Vincenzi, M., Accardo, A., Costantini, S., Scala, S., Portella, L., Trotta, A., Ronga, L., Guillon, J., Leone, M., Colonna, G., Rossi, F., & Tesauro, D. (2015). Intrinsically disordered amphiphilic peptides as potential targets in drug delivery vehicles. Molecular BioSystems, 11(11), 2925-2932. https://doi.org/10.1039/c5mb00358j