Inverse expression of peroxisomes and connexin-43 in the granulosa cells of the quail follicle

Stefano Farioli-Vecchioli, Stefaan Raes, Marc Espeel, Frank Roels, Katharina D'Herde

Research output: Contribution to journalArticle

Abstract

Studying the regulation of peroxisome (Px) expression could improve our understanding of human peroxisomal disorders. The granulosa of the largest preovulatory quail follicles proved to be a relevant model because (a) Px expression changes according to the follicular maturation stage and (b) Px expression varies regionally according to the distance of the granulosa relative to the germinal disc region containing the female gamete (oocyte). The question was asked whether Px expression is related to the extent of metabolic cell coupling and whether zonal Px variation is causally related to oocytal factors. This was evaluated by the presence of catalase and Cx-43 (marker proteins for peroxisomes and gap junctions, respectively) and by in vitro experiments with granulosa explants. The data obtained show that the expression of Cx-43 and Px is inversely correlated both temporally and spatially. Uncoupling of gap junctions results in an upregulation of α- catalase immunofluorescence. This is in agreement with reports that gap junctions are often negatively affected by Px proliferators. The zonal gradient in Px expression appears to be imposed by the oocyte, as is the case for steroidogenesis and proliferative capacity in the granulosa epithelium.

Original languageEnglish
Pages (from-to)167-177
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume48
Issue number2
Publication statusPublished - Feb 2000

Fingerprint

Connexin 43
Quail
Peroxisomes
Granulosa Cells
Gap Junctions
Catalase
Oocytes
Blastodisc
Peroxisomal Disorders
Peroxisome Proliferators
Connexins
Germ Cells
Fluorescent Antibody Technique
Up-Regulation
Epithelium

Keywords

  • Catalase
  • Connexin- 43
  • Gap junctions
  • Granulosa
  • Peroxisomes
  • Regulation
  • Steroidogenesis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Farioli-Vecchioli, S., Raes, S., Espeel, M., Roels, F., & D'Herde, K. (2000). Inverse expression of peroxisomes and connexin-43 in the granulosa cells of the quail follicle. Journal of Histochemistry and Cytochemistry, 48(2), 167-177.

Inverse expression of peroxisomes and connexin-43 in the granulosa cells of the quail follicle. / Farioli-Vecchioli, Stefano; Raes, Stefaan; Espeel, Marc; Roels, Frank; D'Herde, Katharina.

In: Journal of Histochemistry and Cytochemistry, Vol. 48, No. 2, 02.2000, p. 167-177.

Research output: Contribution to journalArticle

Farioli-Vecchioli, S, Raes, S, Espeel, M, Roels, F & D'Herde, K 2000, 'Inverse expression of peroxisomes and connexin-43 in the granulosa cells of the quail follicle', Journal of Histochemistry and Cytochemistry, vol. 48, no. 2, pp. 167-177.
Farioli-Vecchioli, Stefano ; Raes, Stefaan ; Espeel, Marc ; Roels, Frank ; D'Herde, Katharina. / Inverse expression of peroxisomes and connexin-43 in the granulosa cells of the quail follicle. In: Journal of Histochemistry and Cytochemistry. 2000 ; Vol. 48, No. 2. pp. 167-177.
@article{7cec9929885946a09ef866dd935bdbe8,
title = "Inverse expression of peroxisomes and connexin-43 in the granulosa cells of the quail follicle",
abstract = "Studying the regulation of peroxisome (Px) expression could improve our understanding of human peroxisomal disorders. The granulosa of the largest preovulatory quail follicles proved to be a relevant model because (a) Px expression changes according to the follicular maturation stage and (b) Px expression varies regionally according to the distance of the granulosa relative to the germinal disc region containing the female gamete (oocyte). The question was asked whether Px expression is related to the extent of metabolic cell coupling and whether zonal Px variation is causally related to oocytal factors. This was evaluated by the presence of catalase and Cx-43 (marker proteins for peroxisomes and gap junctions, respectively) and by in vitro experiments with granulosa explants. The data obtained show that the expression of Cx-43 and Px is inversely correlated both temporally and spatially. Uncoupling of gap junctions results in an upregulation of α- catalase immunofluorescence. This is in agreement with reports that gap junctions are often negatively affected by Px proliferators. The zonal gradient in Px expression appears to be imposed by the oocyte, as is the case for steroidogenesis and proliferative capacity in the granulosa epithelium.",
keywords = "Catalase, Connexin- 43, Gap junctions, Granulosa, Peroxisomes, Regulation, Steroidogenesis",
author = "Stefano Farioli-Vecchioli and Stefaan Raes and Marc Espeel and Frank Roels and Katharina D'Herde",
year = "2000",
month = "2",
language = "English",
volume = "48",
pages = "167--177",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "2",

}

TY - JOUR

T1 - Inverse expression of peroxisomes and connexin-43 in the granulosa cells of the quail follicle

AU - Farioli-Vecchioli, Stefano

AU - Raes, Stefaan

AU - Espeel, Marc

AU - Roels, Frank

AU - D'Herde, Katharina

PY - 2000/2

Y1 - 2000/2

N2 - Studying the regulation of peroxisome (Px) expression could improve our understanding of human peroxisomal disorders. The granulosa of the largest preovulatory quail follicles proved to be a relevant model because (a) Px expression changes according to the follicular maturation stage and (b) Px expression varies regionally according to the distance of the granulosa relative to the germinal disc region containing the female gamete (oocyte). The question was asked whether Px expression is related to the extent of metabolic cell coupling and whether zonal Px variation is causally related to oocytal factors. This was evaluated by the presence of catalase and Cx-43 (marker proteins for peroxisomes and gap junctions, respectively) and by in vitro experiments with granulosa explants. The data obtained show that the expression of Cx-43 and Px is inversely correlated both temporally and spatially. Uncoupling of gap junctions results in an upregulation of α- catalase immunofluorescence. This is in agreement with reports that gap junctions are often negatively affected by Px proliferators. The zonal gradient in Px expression appears to be imposed by the oocyte, as is the case for steroidogenesis and proliferative capacity in the granulosa epithelium.

AB - Studying the regulation of peroxisome (Px) expression could improve our understanding of human peroxisomal disorders. The granulosa of the largest preovulatory quail follicles proved to be a relevant model because (a) Px expression changes according to the follicular maturation stage and (b) Px expression varies regionally according to the distance of the granulosa relative to the germinal disc region containing the female gamete (oocyte). The question was asked whether Px expression is related to the extent of metabolic cell coupling and whether zonal Px variation is causally related to oocytal factors. This was evaluated by the presence of catalase and Cx-43 (marker proteins for peroxisomes and gap junctions, respectively) and by in vitro experiments with granulosa explants. The data obtained show that the expression of Cx-43 and Px is inversely correlated both temporally and spatially. Uncoupling of gap junctions results in an upregulation of α- catalase immunofluorescence. This is in agreement with reports that gap junctions are often negatively affected by Px proliferators. The zonal gradient in Px expression appears to be imposed by the oocyte, as is the case for steroidogenesis and proliferative capacity in the granulosa epithelium.

KW - Catalase

KW - Connexin- 43

KW - Gap junctions

KW - Granulosa

KW - Peroxisomes

KW - Regulation

KW - Steroidogenesis

UR - http://www.scopus.com/inward/record.url?scp=0034000371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034000371&partnerID=8YFLogxK

M3 - Article

C2 - 10639483

AN - SCOPUS:0034000371

VL - 48

SP - 167

EP - 177

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 2

ER -