Investigating neuroprotective effects of primary glial cells using overflow microfluidic networks

Fabio Bianco, Noemi Tonna, Robert D. Lovchik, Raffaella Morini, Ana Ruiz, Rosa Mastrangelo, Emmanuel Delamarche, Michela Matteoli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The complex intercellular processes within neuroinflammatory events, taking place during brain diseases, are still insufficiently understood. Intercellular pathways between isolated populations of different brain cells need to be studied to unravel the complexity of such events. Here we show how overflow microfluidic networks (oMFNs) can be employed to study the neuroprotective potential of primary astrocytes under the conditions of an ischemic stroke. Astrocytes and neurons were cultivated in two individual chambers of an oMFN and stressed using an oxygen glucose deprivation chamber (OGD). Up to 45% of the neurons died under these conditions. In contrast, neuronal death was significantly reduced to ∼5% when the neurons received fluid from the chamber with the astrocytes culture.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1505-1507
Number of pages3
Volume3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Cellular pathways
  • Microfluidics
  • Neurodegenerative diseases

ASJC Scopus subject areas

  • Control and Systems Engineering

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    Bianco, F., Tonna, N., Lovchik, R. D., Morini, R., Ruiz, A., Mastrangelo, R., Delamarche, E., & Matteoli, M. (2011). Investigating neuroprotective effects of primary glial cells using overflow microfluidic networks. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 3, pp. 1505-1507)