Investigating the mechanical properties of zona pellucida of whole human oocytes by atomic force spectroscopy

Laura Andolfi, Elena Masiero, Elena Giolo, Monica Martinelli, Stefania Luppi, Simone Dal Zilio, Ines Delfino, Roberta Bortul, Marina Zweyer, Giuseppe Ricci, Marco Lazzarino

Research output: Contribution to journalArticle

Abstract

The role of mechanics in numerous biological processes is nowadays recognized, while in others, such as the fertilization process, it is still neglected. In the case of oocytes the description of their mechanical properties could improve the comprehension of the oocyte-spermatozoon interaction and be helpful for application in in vitro fertilization (IVF) clinics. Herein the mechanical properties of whole human oocytes (HOs) immediately after retrieval are investigated by indentation measurements with atomic force spectroscopy under physiological conditions. Measurements are performed on immature (metaphase I-MI) and mature (metaphase II-MII) HOs. According to their morphological characteristics MII-HOs are classified as "suitable" and "rejected"; these latter would be usually rejected for intracytoplasmic sperm injection (ICSI). For all maturation stages we observe that the elastic response of the zona pellucida (ZP) outer layer was different and distinguishable from the rest of the ZP-HO. The elasticity of this ZP outer layer varies with maturation and quality: stiffness decreases from immature MI to good quality MII, up to poor-quality rejected MII. An indirect analysis with IVF outcome indicates that the ZP outer layer of analysed HOs donated by women who achieved pregnancy is stiffer than that of HOs from women with negative outcome. Our findings suggest that mechanical properties can represent important oocyte quality indicators that may be exploited for the design of innovative ICSI dedicated cell sorters.

Original languageEnglish
Pages (from-to)886-893
Number of pages8
JournalIntegrative Biology
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 1 2016

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Zona Pellucida
Oocytes
Spectrum Analysis
Spectroscopy
Mechanical properties
Indentation
Elasticity
Mechanics
Intracytoplasmic Sperm Injections
Stiffness
Fertilization in Vitro
Metaphase
Biological Phenomena
Fertilization
Spermatozoa
Pregnancy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Andolfi, L., Masiero, E., Giolo, E., Martinelli, M., Luppi, S., Dal Zilio, S., ... Lazzarino, M. (2016). Investigating the mechanical properties of zona pellucida of whole human oocytes by atomic force spectroscopy. Integrative Biology, 8(8), 886-893. https://doi.org/10.1039/c6ib00044d

Investigating the mechanical properties of zona pellucida of whole human oocytes by atomic force spectroscopy. / Andolfi, Laura; Masiero, Elena; Giolo, Elena; Martinelli, Monica; Luppi, Stefania; Dal Zilio, Simone; Delfino, Ines; Bortul, Roberta; Zweyer, Marina; Ricci, Giuseppe; Lazzarino, Marco.

In: Integrative Biology, Vol. 8, No. 8, 01.08.2016, p. 886-893.

Research output: Contribution to journalArticle

Andolfi, L, Masiero, E, Giolo, E, Martinelli, M, Luppi, S, Dal Zilio, S, Delfino, I, Bortul, R, Zweyer, M, Ricci, G & Lazzarino, M 2016, 'Investigating the mechanical properties of zona pellucida of whole human oocytes by atomic force spectroscopy', Integrative Biology, vol. 8, no. 8, pp. 886-893. https://doi.org/10.1039/c6ib00044d
Andolfi, Laura ; Masiero, Elena ; Giolo, Elena ; Martinelli, Monica ; Luppi, Stefania ; Dal Zilio, Simone ; Delfino, Ines ; Bortul, Roberta ; Zweyer, Marina ; Ricci, Giuseppe ; Lazzarino, Marco. / Investigating the mechanical properties of zona pellucida of whole human oocytes by atomic force spectroscopy. In: Integrative Biology. 2016 ; Vol. 8, No. 8. pp. 886-893.
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