Investigating the reaction of a novel silica capillary coating compound with proteins/peptides by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

Marina Galvani, Mahmoud Hamdan, Pier Giorgio Righetti, Cecilia Gelfi, Roberto Sebastiano, Attilio Citterio

Research output: Contribution to journalArticlepeer-review

Abstract

Quaternized piperazine ((N-methyl-N-ω-iodobutyl-N'-methyl)piperazine; QPzl) is a novel compound described as an ideal coating material for the silica capillaries that are commonly used for capillary zone electrophoresis. In the course of such analysis, contact between such coatings and biomolecules may result in certain modifications of the latter. To gain specific information on such potential modifications, solutions at pH 10.0 containing both QPzl and standard proteins/peptides were incubated for various periods and examined by matrix-assisted laser desorption/ionisation mass spectrometry. The reduction of the S-S bridges, denaturation in 8 M urea, the isoelectric point of the protein and the duration of the incubation had a profound influence on the investigated reaction. Analysis in reflectron mode and post source decay identified Cys as the likely site of interaction. The implications of the present measurements for proteome analysis using capillary and gel electrophoresis are discussed.

Original languageEnglish
Pages (from-to)210-216
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume15
Issue number3
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Fingerprint

Dive into the research topics of 'Investigating the reaction of a novel silica capillary coating compound with proteins/peptides by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry'. Together they form a unique fingerprint.

Cite this