We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.
|Number of pages||5|
|Publication status||Published - Dec 2002|
- Ligase detection reaction
- Universal array
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology