Investigation of a subset of human polymorphisms by ligase detection reaction and universal array

C. Consolandi; R. Bordoni; B. Castiglioni; A. Frosini; A. Mezzelani; E. Rizzi; C. Battaglia; C. Pera; G. B. Ferrara; P. Zatta; P. Zambenedetti; L. Rossi Bernardi; G. De Bellis

Investigation of a subset of human polymorphisms by ligase detection reaction and universal array

C. Consolandi, R. Bordoni, B. Castiglioni, A. Frosini, A. Mezzelani, E. Rizzi, C. Battaglia, C. Pera, G. B. Ferrara, P. Zatta, P. Zambenedetti, L. Rossi Bernardi, G. De Bellis

IRCCS Multimedica

Research output: Contribution to journal › Article › peer-review

Abstract

We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.

Original language	English
Pages (from-to)	259-263
Number of pages	5
Journal	Minerva Biotecnologica
Volume	14
Issue number	3-4
Publication status	Published - Dec 2002

Keywords

Ligase detection reaction
Polymorphism
Universal array

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology
Bioengineering

Cite this

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title = "Investigation of a subset of human polymorphisms by ligase detection reaction and universal array",

abstract = "We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.",

keywords = "Ligase detection reaction, Polymorphism, Universal array",

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year = "2002",

month = dec,

language = "English",

volume = "14",

pages = "259--263",

journal = "Minerva Biotecnologica",

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T1 - Investigation of a subset of human polymorphisms by ligase detection reaction and universal array

AU - Consolandi, C.

AU - Bordoni, R.

AU - Castiglioni, B.

AU - Frosini, A.

AU - Mezzelani, A.

AU - Rizzi, E.

AU - Battaglia, C.

AU - Pera, C.

AU - Ferrara, G. B.

AU - Zatta, P.

AU - Zambenedetti, P.

AU - Rossi Bernardi, L.

AU - De Bellis, G.

PY - 2002/12

Y1 - 2002/12

N2 - We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.

AB - We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.

KW - Ligase detection reaction

KW - Polymorphism

KW - Universal array

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IS - 3-4

ER -

Investigation of a subset of human polymorphisms by ligase detection reaction and universal array

Abstract

Keywords

ASJC Scopus subject areas

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