Investigation of a subset of human polymorphisms by ligase detection reaction and universal array

C. Consolandi, R. Bordoni, B. Castiglioni, A. Frosini, A. Mezzelani, E. Rizzi, C. Battaglia, C. Pera, G. B. Ferrara, P. Zatta, P. Zambenedetti, L. Rossi Bernardi, G. De Bellis

Research output: Contribution to journalArticle

Abstract

We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalMinerva Biotecnologica
Volume14
Issue number3-4
Publication statusPublished - Dec 2002

Fingerprint

DNA sequences
Ligases
Polymorphism
Exons
DNA
Genes
Throughput
Apolipoprotein E4
Forensic Anthropology
HLA-A Antigens
Feasibility Studies
Transferrin
Oligonucleotide Array Sequence Analysis
DNA Sequence Analysis
Alleles
Polymerase Chain Reaction
Apolipoproteins

Keywords

  • Ligase detection reaction
  • Polymorphism
  • Universal array

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Consolandi, C., Bordoni, R., Castiglioni, B., Frosini, A., Mezzelani, A., Rizzi, E., ... De Bellis, G. (2002). Investigation of a subset of human polymorphisms by ligase detection reaction and universal array. Minerva Biotecnologica, 14(3-4), 259-263.

Investigation of a subset of human polymorphisms by ligase detection reaction and universal array. / Consolandi, C.; Bordoni, R.; Castiglioni, B.; Frosini, A.; Mezzelani, A.; Rizzi, E.; Battaglia, C.; Pera, C.; Ferrara, G. B.; Zatta, P.; Zambenedetti, P.; Rossi Bernardi, L.; De Bellis, G.

In: Minerva Biotecnologica, Vol. 14, No. 3-4, 12.2002, p. 259-263.

Research output: Contribution to journalArticle

Consolandi, C, Bordoni, R, Castiglioni, B, Frosini, A, Mezzelani, A, Rizzi, E, Battaglia, C, Pera, C, Ferrara, GB, Zatta, P, Zambenedetti, P, Rossi Bernardi, L & De Bellis, G 2002, 'Investigation of a subset of human polymorphisms by ligase detection reaction and universal array', Minerva Biotecnologica, vol. 14, no. 3-4, pp. 259-263.
Consolandi C, Bordoni R, Castiglioni B, Frosini A, Mezzelani A, Rizzi E et al. Investigation of a subset of human polymorphisms by ligase detection reaction and universal array. Minerva Biotecnologica. 2002 Dec;14(3-4):259-263.
Consolandi, C. ; Bordoni, R. ; Castiglioni, B. ; Frosini, A. ; Mezzelani, A. ; Rizzi, E. ; Battaglia, C. ; Pera, C. ; Ferrara, G. B. ; Zatta, P. ; Zambenedetti, P. ; Rossi Bernardi, L. ; De Bellis, G. / Investigation of a subset of human polymorphisms by ligase detection reaction and universal array. In: Minerva Biotecnologica. 2002 ; Vol. 14, No. 3-4. pp. 259-263.
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