Abstract
We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.
| Original language | English |
|---|---|
| Pages (from-to) | 259-263 |
| Number of pages | 5 |
| Journal | Minerva Biotecnologica |
| Volume | 14 |
| Issue number | 3-4 |
| Publication status | Published - Dec 2002 |
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Keywords
- Ligase detection reaction
- Polymorphism
- Universal array
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Investigation of a subset of human polymorphisms by ligase detection reaction and universal array. / Consolandi, C.; Bordoni, R.; Castiglioni, B.; Frosini, A.; Mezzelani, A.; Rizzi, E.; Battaglia, C.; Pera, C.; Ferrara, G. B.; Zatta, P.; Zambenedetti, P.; Rossi Bernardi, L.; De Bellis, G.
In: Minerva Biotecnologica, Vol. 14, No. 3-4, 12.2002, p. 259-263.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Investigation of a subset of human polymorphisms by ligase detection reaction and universal array
AU - Consolandi, C.
AU - Bordoni, R.
AU - Castiglioni, B.
AU - Frosini, A.
AU - Mezzelani, A.
AU - Rizzi, E.
AU - Battaglia, C.
AU - Pera, C.
AU - Ferrara, G. B.
AU - Zatta, P.
AU - Zambenedetti, P.
AU - Rossi Bernardi, L.
AU - De Bellis, G.
PY - 2002/12
Y1 - 2002/12
N2 - We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.
AB - We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.
KW - Ligase detection reaction
KW - Polymorphism
KW - Universal array
UR - http://www.scopus.com/inward/record.url?scp=0036977929&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036977929&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0036977929
VL - 14
SP - 259
EP - 263
JO - Minerva Biotecnologica
JF - Minerva Biotecnologica
SN - 1120-4826
IS - 3-4
ER -