Abstract
We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.
Original language | English |
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Pages (from-to) | 259-263 |
Number of pages | 5 |
Journal | Minerva Biotecnologica |
Volume | 14 |
Issue number | 3-4 |
Publication status | Published - Dec 2002 |
Keywords
- Ligase detection reaction
- Polymorphism
- Universal array
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering