Investigation of interactions between poly-l-lysine-coated boron nitride nanotubes and c2c12 cells: Up-take, cytocompatibility, and differentiation

G. Ciofani, L. Ricotti, S. Danti, S. Moscato, C. Nesti, D. D'Alessandro, D. Dinucci, F. Chiellini, A. Pietrabissa, M. Petrini, A. Menciassi

Research output: Contribution to journalArticle

Abstract

Boron nitride nanotubes (BNNTs) have generated considerable interest within the scientific community by virtue of their unique physical properties, which can be exploited in the biomedical field. In the present in vitro study, we investigated the interactions of poly-l-lysine-coated BNNTs with C2C12 cells, as a model of muscle cells, in terms of cytocompatibility and BNNT internalization. The latter was performed using both confocal and transmission electron microscopy. Finally, we investigated myoblast differentiation in the presence of BNNTs, evaluating the protein synthesis of differentiating cells, myotube formation, and expression of some constitutive myoblastic markers, such as MyoD and Cx43, by reverse transcription - polymerase chain reaction and Western blot analysis. We demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2C12 myoblast viability nor significantly interfering with myotube formation.

Original languageEnglish
Pages (from-to)285-298
Number of pages14
JournalInternational Journal of Nanomedicine
Volume5
Issue number1
Publication statusPublished - 2010

    Fingerprint

Keywords

  • Boron nitride nanotubes
  • C2C12 cells
  • Connexin 43
  • Cytocompatibility
  • Differentiation
  • MyoD
  • Up-take

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Ciofani, G., Ricotti, L., Danti, S., Moscato, S., Nesti, C., D'Alessandro, D., Dinucci, D., Chiellini, F., Pietrabissa, A., Petrini, M., & Menciassi, A. (2010). Investigation of interactions between poly-l-lysine-coated boron nitride nanotubes and c2c12 cells: Up-take, cytocompatibility, and differentiation. International Journal of Nanomedicine, 5(1), 285-298.