Investigation of synaptic microcircuits using patch-clamp paired recordings in acute brain slices

Giovanni Russo, Stefano Taverna

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Multiple patch clamp recordings represent a powerful tool to investigate functional synaptic connectivity among individual cells. This technical approach is particularly useful to study the synaptic organization of microcircuits in certain brain areas, e.g., the striatum, which are characterized by heterogeneous cell populations and an apparent lack of an anatomically ordered cytoarchitecture. Fast-spiking interneurons (FSIs) represent less than 1 % of striatal neurons, but despite their rareness they exert a strong influence on signal processing by striatal microcircuits and principal cells output. Little is known about the functional properties of chemical and electrical synapses connecting striatal FSIs to each other. Here we describe a simple dual patch clamp approach to investigate electrical and GABAergic synapses in mouse neostriatal slices.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages183-193
Number of pages11
Volume1183
ISBN (Print)9781493910953
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1183
ISSN (Print)10643745

Fingerprint

Corpus Striatum
Electrical Synapses
Interneurons
Brain
Neurons
Population

Keywords

  • Dual patch-clamp
  • GABA
  • Gap junctions
  • IPSC
  • Striatum
  • Synaptic connectivity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Russo, G., & Taverna, S. (2014). Investigation of synaptic microcircuits using patch-clamp paired recordings in acute brain slices. In Methods in Molecular Biology (Vol. 1183, pp. 183-193). (Methods in Molecular Biology; Vol. 1183). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-1096-0_11

Investigation of synaptic microcircuits using patch-clamp paired recordings in acute brain slices. / Russo, Giovanni; Taverna, Stefano.

Methods in Molecular Biology. Vol. 1183 Humana Press Inc., 2014. p. 183-193 (Methods in Molecular Biology; Vol. 1183).

Research output: Chapter in Book/Report/Conference proceedingChapter

Russo, G & Taverna, S 2014, Investigation of synaptic microcircuits using patch-clamp paired recordings in acute brain slices. in Methods in Molecular Biology. vol. 1183, Methods in Molecular Biology, vol. 1183, Humana Press Inc., pp. 183-193. https://doi.org/10.1007/978-1-4939-1096-0_11
Russo G, Taverna S. Investigation of synaptic microcircuits using patch-clamp paired recordings in acute brain slices. In Methods in Molecular Biology. Vol. 1183. Humana Press Inc. 2014. p. 183-193. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-1096-0_11
Russo, Giovanni ; Taverna, Stefano. / Investigation of synaptic microcircuits using patch-clamp paired recordings in acute brain slices. Methods in Molecular Biology. Vol. 1183 Humana Press Inc., 2014. pp. 183-193 (Methods in Molecular Biology).
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