Investigation of the hybridization process in DNA-microarrays by fluorescence lifetime imaging

Daniela Comelli, Cosimo D'Andrea, Paola Taroni, Gianluca Valentini, Rinaldo Cubeddu, Clarissa Consolandi, Gianluca De Bellis, Luigi Rossi Bernardi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

cDNA-microarrays for expression profiling rely on the assumption that the fluorescence signal is linearly proportional to the concentration of the markers. Yet, interactions amongst different markers and with the substrate might affect the chromophore quantum yield and lead to an erroneous reading. To indirectly evaluate quantum yield changes and their relevance for DNA-microarray reading, we studied the effect on fluorescence lifetime by using a system for fluorescence lifetime imaging. Quenching of the fluorescence emission was observed in the tight environment represented by a DNA-microarray due to concentration and to interactions with the processing chemicals present on the substrate.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsG.A. Wagnieres
Pages81-87
Number of pages7
Volume5141
Publication statusPublished - 2003
EventDiagnostic Optical Spectroscopy in Biomedicine II - Munich, Germany
Duration: Jun 24 2003Jun 25 2003

Other

OtherDiagnostic Optical Spectroscopy in Biomedicine II
CountryGermany
CityMunich
Period6/24/036/25/03

Keywords

  • Fluorescence lifetime
  • Fluorescent biosensors
  • Time-resolved imaging

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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