Investigation of the properties of novel acrylamido monomers by capillary zone electrophoresis

Cecilia Gelfi, Patrizia de Besi, Angela Alloni, Pier Giorgio Righetti

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A series of mono- and disubstituted acrylamide monomers [monomethylacrylamide, dimethylacrylamide, trisacryl (Tris-A), dideoxy- trisacryl and acryloylmorpholine] were investigated as potential candidates of a novel class of polyacrylamide matrices, exhibiting high hydrophilicity, high resistance to hydrolysis and larger pore size than conventional polyacrylamide gels. However, the most promising monomer (Tris-A) exhibited first-order degradation kinetics in 0.1 M NaOH, suggesting that such a structure is electronically unstable. On the other hand, the fact that, once incorporated into a polymer chain, such a monomer exhibited a strong resistance to alkaline hydrolysis suggests that, perhaps, a poly(trisacryl) matrix could be stereoregular, perhaps via helix formation. Another unique finding is the inverse relationship between the partition coefficient of such monomers and the incorporation efficiency: the more hydrophobic members of the family exhibit a very poor conversion from monomer into polymer. The efficiency, however, can be dramatically increased by increasing the polymerization temperature from 25 to 60°C.

Original languageEnglish
Pages (from-to)333-341
Number of pages9
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - Sep 11 1992

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

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