TY - JOUR
T1 - Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG)
AU - Putignani, L.
AU - Mancinelli, L.
AU - Chierico, F. Del
AU - Menichella, D.
AU - Adlerstein, D.
AU - Angelici, M. C.
AU - Marangi, M.
AU - Berrilli, F.
AU - Caffara, M.
AU - Regalbono, D. A Frangipane di
AU - Giangaspero, A.
PY - 2011/2
Y1 - 2011/2
N2 - To evaluate the presence of Toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 Crassostrea gigas (6 pools), 660 Mytilus galloprovincialis (22 pools), 804 Tapes decussatus (28 pools) and 161 Tapes philippinarum (6 pools), were collected from the Varano Lagoon (Apulia, Italy).Shellfish from 62 pools were subjected to two molecular techniques: a nested-PCR assay, and a fluorescent amplicon generation (FLAG) real-time PCR assay, both based on the multi-copy B1 target, were performed. One pooled sample of gills from C. gigas and one pooled sample of haemolymphs from T. decussatus were assessed as positive for T. gondii DNA by both techniques. The results demonstrated the presence of T. gondii in edible farmed C. gigas and T. decussatus and indicate that there may be a considerable health threat involved in eating contaminated raw shellfish.
AB - To evaluate the presence of Toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 Crassostrea gigas (6 pools), 660 Mytilus galloprovincialis (22 pools), 804 Tapes decussatus (28 pools) and 161 Tapes philippinarum (6 pools), were collected from the Varano Lagoon (Apulia, Italy).Shellfish from 62 pools were subjected to two molecular techniques: a nested-PCR assay, and a fluorescent amplicon generation (FLAG) real-time PCR assay, both based on the multi-copy B1 target, were performed. One pooled sample of gills from C. gigas and one pooled sample of haemolymphs from T. decussatus were assessed as positive for T. gondii DNA by both techniques. The results demonstrated the presence of T. gondii in edible farmed C. gigas and T. decussatus and indicate that there may be a considerable health threat involved in eating contaminated raw shellfish.
KW - Edible shellfish
KW - FLAG real-time PCR
KW - Italy
KW - Nested-PCR
KW - Toxoplama gondii
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UR - http://www.scopus.com/inward/citedby.url?scp=78751580315&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2010.09.007
DO - 10.1016/j.exppara.2010.09.007
M3 - Article
C2 - 20920501
AN - SCOPUS:78751580315
VL - 127
SP - 409
EP - 417
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 2
ER -