Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG)

L. Putignani; L. Mancinelli; F. Del Chierico; D. Menichella; D. Adlerstein; M. C. Angelici; M. Marangi; F. Berrilli; M. Caffara; D. A Frangipane di Regalbono; A. Giangaspero

doi:10.1016/j.exppara.2010.09.007

Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG)

L. Putignani, L. Mancinelli, F. Del Chierico, D. Menichella, D. Adlerstein, M. C. Angelici, M. Marangi, F. Berrilli, M. Caffara, D. A Frangipane di Regalbono, A. Giangaspero

Ospedale Pediatrico Bambino Gesu

Research output: Contribution to journal › Article › peer-review

Abstract

To evaluate the presence of Toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 Crassostrea gigas (6 pools), 660 Mytilus galloprovincialis (22 pools), 804 Tapes decussatus (28 pools) and 161 Tapes philippinarum (6 pools), were collected from the Varano Lagoon (Apulia, Italy).Shellfish from 62 pools were subjected to two molecular techniques: a nested-PCR assay, and a fluorescent amplicon generation (FLAG) real-time PCR assay, both based on the multi-copy B1 target, were performed. One pooled sample of gills from C. gigas and one pooled sample of haemolymphs from T. decussatus were assessed as positive for T. gondii DNA by both techniques. The results demonstrated the presence of T. gondii in edible farmed C. gigas and T. decussatus and indicate that there may be a considerable health threat involved in eating contaminated raw shellfish.

Original language	English
Pages (from-to)	409-417
Number of pages	9
Journal	Experimental Parasitology
Volume	127
Issue number	2
DOIs	https://doi.org/10.1016/j.exppara.2010.09.007
Publication status	Published - Feb 2011

Keywords

Edible shellfish
FLAG real-time PCR
Italy
Nested-PCR
Toxoplama gondii

ASJC Scopus subject areas

Parasitology
Immunology

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10.1016/j.exppara.2010.09.007

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Putignani, L., Mancinelli, L., Chierico, F. D., Menichella, D., Adlerstein, D., Angelici, M. C., Marangi, M., Berrilli, F., Caffara, M., Regalbono, D. A. F. D., & Giangaspero, A. (2011). Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG). Experimental Parasitology, 127(2), 409-417. https://doi.org/10.1016/j.exppara.2010.09.007

Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG). / Putignani, L.; Mancinelli, L.; Chierico, F. Del; Menichella, D.; Adlerstein, D.; Angelici, M. C.; Marangi, M.; Berrilli, F.; Caffara, M.; Regalbono, D. A Frangipane di; Giangaspero, A.

In: Experimental Parasitology, Vol. 127, No. 2, 02.2011, p. 409-417.

Research output: Contribution to journal › Article › peer-review

Putignani, L, Mancinelli, L, Chierico, FD, Menichella, D, Adlerstein, D, Angelici, MC, Marangi, M, Berrilli, F, Caffara, M, Regalbono, DAFD & Giangaspero, A 2011, 'Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG)', Experimental Parasitology, vol. 127, no. 2, pp. 409-417. https://doi.org/10.1016/j.exppara.2010.09.007

Putignani, L. ; Mancinelli, L. ; Chierico, F. Del ; Menichella, D. ; Adlerstein, D. ; Angelici, M. C. ; Marangi, M. ; Berrilli, F. ; Caffara, M. ; Regalbono, D. A Frangipane di ; Giangaspero, A. / Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG). In: Experimental Parasitology. 2011 ; Vol. 127, No. 2. pp. 409-417.

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abstract = "To evaluate the presence of Toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 Crassostrea gigas (6 pools), 660 Mytilus galloprovincialis (22 pools), 804 Tapes decussatus (28 pools) and 161 Tapes philippinarum (6 pools), were collected from the Varano Lagoon (Apulia, Italy).Shellfish from 62 pools were subjected to two molecular techniques: a nested-PCR assay, and a fluorescent amplicon generation (FLAG) real-time PCR assay, both based on the multi-copy B1 target, were performed. One pooled sample of gills from C. gigas and one pooled sample of haemolymphs from T. decussatus were assessed as positive for T. gondii DNA by both techniques. The results demonstrated the presence of T. gondii in edible farmed C. gigas and T. decussatus and indicate that there may be a considerable health threat involved in eating contaminated raw shellfish.",

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Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG)

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Keywords

ASJC Scopus subject areas

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