Involvement of 5-lipoxygenase in Alzheimer's disease: A role for DNA methylation

Andrea Di Francesco; Beatrice Arosio; Cristina Gussago; Enrico Dainese; Daniela Mari; Claudio D'Addario; Mauro MacCarrone

doi:10.3233/JAD-130506

Involvement of 5-lipoxygenase in Alzheimer's disease: A role for DNA methylation

Andrea Di Francesco, Beatrice Arosio, Cristina Gussago, Enrico Dainese, Daniela Mari, Claudio D'Addario, Mauro MacCarrone

Research output: Contribution to journal › Article › peer-review

Abstract

Lipoxygenases play a major role in the neuropathology of Alzheimer's disease (AD), even though the underlying mechanisms are as yet poorly understood. Here, we studied the epigenetic regulation of 5-lipoxygenase (5-LOX) in peripheral blood mononuclear cells of subjects with late-onset AD and age-matched controls. We found a significant increase in 5-LOX gene expression in AD subjects compared to healthy controls, paralleled by increased 5-LOX protein and leukotriene B₄, the 5-LOX product. In addition, a consistent reduction in DNA methylation at 5-LOX gene promoter was documented in AD versus healthy subjects. Taken together, our findings further support a role for 5-LOX in vulnerability to neurodegeneration.

Original language	English
Pages (from-to)	3-8
Number of pages	6
Journal	Journal of Alzheimer's Disease
Volume	37
Issue number	1
DOIs	https://doi.org/10.3233/JAD-130506
Publication status	Published - 2013

Keywords

5-lipoxygenase
Alzheimer's disease
biomarker
DNA methylation
fatty acid amide hydrolase
peripheral blood mononuclear cells

ASJC Scopus subject areas

Psychiatry and Mental health
Geriatrics and Gerontology
Clinical Psychology

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10.3233/JAD-130506

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title = "Involvement of 5-lipoxygenase in Alzheimer's disease: A role for DNA methylation",

abstract = "Lipoxygenases play a major role in the neuropathology of Alzheimer's disease (AD), even though the underlying mechanisms are as yet poorly understood. Here, we studied the epigenetic regulation of 5-lipoxygenase (5-LOX) in peripheral blood mononuclear cells of subjects with late-onset AD and age-matched controls. We found a significant increase in 5-LOX gene expression in AD subjects compared to healthy controls, paralleled by increased 5-LOX protein and leukotriene B4, the 5-LOX product. In addition, a consistent reduction in DNA methylation at 5-LOX gene promoter was documented in AD versus healthy subjects. Taken together, our findings further support a role for 5-LOX in vulnerability to neurodegeneration.",

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author = "{Di Francesco}, Andrea and Beatrice Arosio and Cristina Gussago and Enrico Dainese and Daniela Mari and Claudio D'Addario and Mauro MacCarrone",

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T1 - Involvement of 5-lipoxygenase in Alzheimer's disease

T2 - A role for DNA methylation

AU - Di Francesco, Andrea

AU - Arosio, Beatrice

AU - Gussago, Cristina

AU - Dainese, Enrico

AU - Mari, Daniela

AU - D'Addario, Claudio

AU - MacCarrone, Mauro

PY - 2013

Y1 - 2013

N2 - Lipoxygenases play a major role in the neuropathology of Alzheimer's disease (AD), even though the underlying mechanisms are as yet poorly understood. Here, we studied the epigenetic regulation of 5-lipoxygenase (5-LOX) in peripheral blood mononuclear cells of subjects with late-onset AD and age-matched controls. We found a significant increase in 5-LOX gene expression in AD subjects compared to healthy controls, paralleled by increased 5-LOX protein and leukotriene B4, the 5-LOX product. In addition, a consistent reduction in DNA methylation at 5-LOX gene promoter was documented in AD versus healthy subjects. Taken together, our findings further support a role for 5-LOX in vulnerability to neurodegeneration.

AB - Lipoxygenases play a major role in the neuropathology of Alzheimer's disease (AD), even though the underlying mechanisms are as yet poorly understood. Here, we studied the epigenetic regulation of 5-lipoxygenase (5-LOX) in peripheral blood mononuclear cells of subjects with late-onset AD and age-matched controls. We found a significant increase in 5-LOX gene expression in AD subjects compared to healthy controls, paralleled by increased 5-LOX protein and leukotriene B4, the 5-LOX product. In addition, a consistent reduction in DNA methylation at 5-LOX gene promoter was documented in AD versus healthy subjects. Taken together, our findings further support a role for 5-LOX in vulnerability to neurodegeneration.

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KW - fatty acid amide hydrolase

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Involvement of 5-lipoxygenase in Alzheimer's disease: A role for DNA methylation

Abstract

Keywords

ASJC Scopus subject areas

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