Involvement of 5-lipoxygenase in spinal cord injury

Tiziana Genovese, Emanuela Mazzon, Antonietta Rossi, Rosanna Di Paola, Giuseppe Cannavò, Carmelo Muià, Concetta Crisafulli, Placido Bramanti, Lidia Sautebin, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

Abstract

A traumatic spinal cord injury (SCI) induces a sequelae of events which conduce biochemical and cellular alterations. Here we compare the degree of spinal cord injury caused by the application of vascular clips, in mice lacking the 5-lipoxygenase and in the corresponding wild-type mice. Biochemical, immunohistochemical and functional studies revealed respectively an increase of neutrophils infiltration, of IL-1β, TNF-α immunoreactivity, apoptosis (measured by Annexin-V staining) and loss of hind legs movement in SCI operated 5-LO wild-type mice. In contrast, the degree of (1) neutrophil infiltration at different time points, (2) cytokine expression (TNF-α and IL-1β), (3) histological damage, (4) apoptosis, was markedly reduced in the tissues obtained from SCI operated 5-LO deficient mice and (5) the motor recovery was ameliorated.

Original languageEnglish
Pages (from-to)55-64
Number of pages10
JournalJournal of Neuroimmunology
Volume166
Issue number1-2
DOIs
Publication statusPublished - Sep 2005

Keywords

  • 5-lipoxygenase
  • Apoptosis
  • Cytokines
  • Histopathology
  • Neutrophil infiltration
  • SCI

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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  • Cite this

    Genovese, T., Mazzon, E., Rossi, A., Di Paola, R., Cannavò, G., Muià, C., Crisafulli, C., Bramanti, P., Sautebin, L., & Cuzzocrea, S. (2005). Involvement of 5-lipoxygenase in spinal cord injury. Journal of Neuroimmunology, 166(1-2), 55-64. https://doi.org/10.1016/j.jneuroim.2005.05.009