Involvement of 5-lipoxygenase in spinal cord injury

Tiziana Genovese, Emanuela Mazzon, Antonietta Rossi, Rosanna Di Paola, Giuseppe Cannavò, Carmelo Muià, Concetta Crisafulli, Placido Bramanti, Lidia Sautebin, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

Abstract

A traumatic spinal cord injury (SCI) induces a sequelae of events which conduce biochemical and cellular alterations. Here we compare the degree of spinal cord injury caused by the application of vascular clips, in mice lacking the 5-lipoxygenase and in the corresponding wild-type mice. Biochemical, immunohistochemical and functional studies revealed respectively an increase of neutrophils infiltration, of IL-1β, TNF-α immunoreactivity, apoptosis (measured by Annexin-V staining) and loss of hind legs movement in SCI operated 5-LO wild-type mice. In contrast, the degree of (1) neutrophil infiltration at different time points, (2) cytokine expression (TNF-α and IL-1β), (3) histological damage, (4) apoptosis, was markedly reduced in the tissues obtained from SCI operated 5-LO deficient mice and (5) the motor recovery was ameliorated.

Original languageEnglish
Pages (from-to)55-64
Number of pages10
JournalJournal of Neuroimmunology
Volume166
Issue number1-2
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Arachidonate 5-Lipoxygenase
Spinal Cord Injuries
Neutrophil Infiltration
Interleukin-1
Apoptosis
Interleukin-3
Annexin A5
Surgical Instruments
Blood Vessels
Leg
Staining and Labeling
Cytokines

Keywords

  • 5-lipoxygenase
  • Apoptosis
  • Cytokines
  • Histopathology
  • Neutrophil infiltration
  • SCI

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Genovese, T., Mazzon, E., Rossi, A., Di Paola, R., Cannavò, G., Muià, C., ... Cuzzocrea, S. (2005). Involvement of 5-lipoxygenase in spinal cord injury. Journal of Neuroimmunology, 166(1-2), 55-64. https://doi.org/10.1016/j.jneuroim.2005.05.009

Involvement of 5-lipoxygenase in spinal cord injury. / Genovese, Tiziana; Mazzon, Emanuela; Rossi, Antonietta; Di Paola, Rosanna; Cannavò, Giuseppe; Muià, Carmelo; Crisafulli, Concetta; Bramanti, Placido; Sautebin, Lidia; Cuzzocrea, Salvatore.

In: Journal of Neuroimmunology, Vol. 166, No. 1-2, 09.2005, p. 55-64.

Research output: Contribution to journalArticle

Genovese, T, Mazzon, E, Rossi, A, Di Paola, R, Cannavò, G, Muià, C, Crisafulli, C, Bramanti, P, Sautebin, L & Cuzzocrea, S 2005, 'Involvement of 5-lipoxygenase in spinal cord injury', Journal of Neuroimmunology, vol. 166, no. 1-2, pp. 55-64. https://doi.org/10.1016/j.jneuroim.2005.05.009
Genovese T, Mazzon E, Rossi A, Di Paola R, Cannavò G, Muià C et al. Involvement of 5-lipoxygenase in spinal cord injury. Journal of Neuroimmunology. 2005 Sep;166(1-2):55-64. https://doi.org/10.1016/j.jneuroim.2005.05.009
Genovese, Tiziana ; Mazzon, Emanuela ; Rossi, Antonietta ; Di Paola, Rosanna ; Cannavò, Giuseppe ; Muià, Carmelo ; Crisafulli, Concetta ; Bramanti, Placido ; Sautebin, Lidia ; Cuzzocrea, Salvatore. / Involvement of 5-lipoxygenase in spinal cord injury. In: Journal of Neuroimmunology. 2005 ; Vol. 166, No. 1-2. pp. 55-64.
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