TY - JOUR
T1 - Involvement of a ceramide activated protein phosphatase in the differentiation of neuroblastoma Neuro2a cells
AU - Prinetti, Alessandro
AU - Bassi, Rosaria
AU - Riboni, Laura
AU - Tettamanti, Guido
PY - 1997/9/8
Y1 - 1997/9/8
N2 - The possible involvement of protein phosphatase in ceramide-mediated neural cell differentiation was investigated. Neuroblastoma Neuro2a cell differentiation induced by retinoic acid, or conditions causing an increase in cellular ceramide, was significantly inhibited by the serine/threonine phosphatase inhibitor okadaic acid, at concentrations as low as 2.5 nM. A crude cytosolic preparation front Neuro2a cells was found to have a cation-independent protein phosphatase activity that was stimulated by ceramide in a dose-dependent manner. Short- and long-chain ceramides, but not sphingosine and related dihydroderivatives, were active. Ceramide-activated protein phosphatase activity from Neuro2a cells was inhibited by 5 nM okadaic acid. The data indicate that a type 2A protein phosphatase is involved in ceramide-mediated differentiation of Neuro2a cells.
AB - The possible involvement of protein phosphatase in ceramide-mediated neural cell differentiation was investigated. Neuroblastoma Neuro2a cell differentiation induced by retinoic acid, or conditions causing an increase in cellular ceramide, was significantly inhibited by the serine/threonine phosphatase inhibitor okadaic acid, at concentrations as low as 2.5 nM. A crude cytosolic preparation front Neuro2a cells was found to have a cation-independent protein phosphatase activity that was stimulated by ceramide in a dose-dependent manner. Short- and long-chain ceramides, but not sphingosine and related dihydroderivatives, were active. Ceramide-activated protein phosphatase activity from Neuro2a cells was inhibited by 5 nM okadaic acid. The data indicate that a type 2A protein phosphatase is involved in ceramide-mediated differentiation of Neuro2a cells.
KW - Ceramide
KW - Neural cell differentiation
KW - Neuroblastoma cells
KW - Protein phosphates
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U2 - 10.1016/S0014-5793(97)00981-2
DO - 10.1016/S0014-5793(97)00981-2
M3 - Article
C2 - 9315744
AN - SCOPUS:0031559915
VL - 414
SP - 475
EP - 479
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -