Abstract
The possible involvement of protein phosphatase in ceramide-mediated neural cell differentiation was investigated. Neuroblastoma Neuro2a cell differentiation induced by retinoic acid, or conditions causing an increase in cellular ceramide, was significantly inhibited by the serine/threonine phosphatase inhibitor okadaic acid, at concentrations as low as 2.5 nM. A crude cytosolic preparation front Neuro2a cells was found to have a cation-independent protein phosphatase activity that was stimulated by ceramide in a dose-dependent manner. Short- and long-chain ceramides, but not sphingosine and related dihydroderivatives, were active. Ceramide-activated protein phosphatase activity from Neuro2a cells was inhibited by 5 nM okadaic acid. The data indicate that a type 2A protein phosphatase is involved in ceramide-mediated differentiation of Neuro2a cells.
| Original language | English |
|---|---|
| Pages (from-to) | 475-479 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 414 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 8 1997 |
Fingerprint
Keywords
- Ceramide
- Neural cell differentiation
- Neuroblastoma cells
- Protein phosphates
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Involvement of a ceramide activated protein phosphatase in the differentiation of neuroblastoma Neuro2a cells. / Prinetti, Alessandro; Bassi, Rosaria; Riboni, Laura; Tettamanti, Guido.
In: FEBS Letters, Vol. 414, No. 2, 08.09.1997, p. 475-479.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Involvement of a ceramide activated protein phosphatase in the differentiation of neuroblastoma Neuro2a cells
AU - Prinetti, Alessandro
AU - Bassi, Rosaria
AU - Riboni, Laura
AU - Tettamanti, Guido
PY - 1997/9/8
Y1 - 1997/9/8
N2 - The possible involvement of protein phosphatase in ceramide-mediated neural cell differentiation was investigated. Neuroblastoma Neuro2a cell differentiation induced by retinoic acid, or conditions causing an increase in cellular ceramide, was significantly inhibited by the serine/threonine phosphatase inhibitor okadaic acid, at concentrations as low as 2.5 nM. A crude cytosolic preparation front Neuro2a cells was found to have a cation-independent protein phosphatase activity that was stimulated by ceramide in a dose-dependent manner. Short- and long-chain ceramides, but not sphingosine and related dihydroderivatives, were active. Ceramide-activated protein phosphatase activity from Neuro2a cells was inhibited by 5 nM okadaic acid. The data indicate that a type 2A protein phosphatase is involved in ceramide-mediated differentiation of Neuro2a cells.
AB - The possible involvement of protein phosphatase in ceramide-mediated neural cell differentiation was investigated. Neuroblastoma Neuro2a cell differentiation induced by retinoic acid, or conditions causing an increase in cellular ceramide, was significantly inhibited by the serine/threonine phosphatase inhibitor okadaic acid, at concentrations as low as 2.5 nM. A crude cytosolic preparation front Neuro2a cells was found to have a cation-independent protein phosphatase activity that was stimulated by ceramide in a dose-dependent manner. Short- and long-chain ceramides, but not sphingosine and related dihydroderivatives, were active. Ceramide-activated protein phosphatase activity from Neuro2a cells was inhibited by 5 nM okadaic acid. The data indicate that a type 2A protein phosphatase is involved in ceramide-mediated differentiation of Neuro2a cells.
KW - Ceramide
KW - Neural cell differentiation
KW - Neuroblastoma cells
KW - Protein phosphates
UR - http://www.scopus.com/inward/record.url?scp=0031559915&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031559915&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(97)00981-2
DO - 10.1016/S0014-5793(97)00981-2
M3 - Article
C2 - 9315744
AN - SCOPUS:0031559915
VL - 414
SP - 475
EP - 479
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -