Involvement of Alu sequences in the cell-specific regulation of transcription of the γ chain of Fc and T cell receptors

A. T. Brini, G. M. Lee, J. P. Kinet

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The FcεRI-γ chains are expressed in a variety of hematopoietic cells where they play a critical role in signal transduction. They are part of the high affinity IgE receptor in mast cells, basophils, Langerhans cells, and possibly other cells; a component of the low affinity receptor for IgG (FcγRIIIA or CD16) in natural killer cells and macrophages; and part of the T cell antigen receptor in subsets of T cells. Here we have investigated the transcriptional regulation of the γ chain gene by analyzing the 2.5-kilobase sequence upstream of the transcription start site. This sequence contains a promoter specific to cells of hematopoietic lineage. However, the tissue specificity of this promoter is only partial because it is active in all of the hematopoietic cells tested here, regardless of whether they constitutively express FcεRI- γ chain transcripts. We have identified two adjacent cis-acting regulatory elements, both of which are part of an Alu repeat. The first (-445/-366) is a positive element active in both basophils and T cells. The second (-365/-264) binds to nuclear factors, which appear to be different in basophils and T cells, and acts as a negative element in basophils and as a positive one in T cells. Thus, this Alu repeat (90% identical to Alu consensus sequences) has evolved to become both a positive and negative regulator.

Original languageEnglish
Pages (from-to)1355-1361
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number2
Publication statusPublished - 1993

Fingerprint

T-cells
Basophils
Transcription
T-Cell Antigen Receptor
T-Lymphocytes
IgG Receptors
IgE Receptors
Signal transduction
Organ Specificity
Langerhans Cells
Macrophages
Transcription Initiation Site
Consensus Sequence
T-Lymphocyte Subsets
Cell Lineage
Mast Cells
Natural Killer Cells
Signal Transduction
Genes
Tissue

ASJC Scopus subject areas

  • Biochemistry

Cite this

Involvement of Alu sequences in the cell-specific regulation of transcription of the γ chain of Fc and T cell receptors. / Brini, A. T.; Lee, G. M.; Kinet, J. P.

In: Journal of Biological Chemistry, Vol. 268, No. 2, 1993, p. 1355-1361.

Research output: Contribution to journalArticle

@article{6e8f16a9ae654246bf2a9f9489d39540,
title = "Involvement of Alu sequences in the cell-specific regulation of transcription of the γ chain of Fc and T cell receptors",
abstract = "The FcεRI-γ chains are expressed in a variety of hematopoietic cells where they play a critical role in signal transduction. They are part of the high affinity IgE receptor in mast cells, basophils, Langerhans cells, and possibly other cells; a component of the low affinity receptor for IgG (FcγRIIIA or CD16) in natural killer cells and macrophages; and part of the T cell antigen receptor in subsets of T cells. Here we have investigated the transcriptional regulation of the γ chain gene by analyzing the 2.5-kilobase sequence upstream of the transcription start site. This sequence contains a promoter specific to cells of hematopoietic lineage. However, the tissue specificity of this promoter is only partial because it is active in all of the hematopoietic cells tested here, regardless of whether they constitutively express FcεRI- γ chain transcripts. We have identified two adjacent cis-acting regulatory elements, both of which are part of an Alu repeat. The first (-445/-366) is a positive element active in both basophils and T cells. The second (-365/-264) binds to nuclear factors, which appear to be different in basophils and T cells, and acts as a negative element in basophils and as a positive one in T cells. Thus, this Alu repeat (90{\%} identical to Alu consensus sequences) has evolved to become both a positive and negative regulator.",
author = "Brini, {A. T.} and Lee, {G. M.} and Kinet, {J. P.}",
year = "1993",
language = "English",
volume = "268",
pages = "1355--1361",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "2",

}

TY - JOUR

T1 - Involvement of Alu sequences in the cell-specific regulation of transcription of the γ chain of Fc and T cell receptors

AU - Brini, A. T.

AU - Lee, G. M.

AU - Kinet, J. P.

PY - 1993

Y1 - 1993

N2 - The FcεRI-γ chains are expressed in a variety of hematopoietic cells where they play a critical role in signal transduction. They are part of the high affinity IgE receptor in mast cells, basophils, Langerhans cells, and possibly other cells; a component of the low affinity receptor for IgG (FcγRIIIA or CD16) in natural killer cells and macrophages; and part of the T cell antigen receptor in subsets of T cells. Here we have investigated the transcriptional regulation of the γ chain gene by analyzing the 2.5-kilobase sequence upstream of the transcription start site. This sequence contains a promoter specific to cells of hematopoietic lineage. However, the tissue specificity of this promoter is only partial because it is active in all of the hematopoietic cells tested here, regardless of whether they constitutively express FcεRI- γ chain transcripts. We have identified two adjacent cis-acting regulatory elements, both of which are part of an Alu repeat. The first (-445/-366) is a positive element active in both basophils and T cells. The second (-365/-264) binds to nuclear factors, which appear to be different in basophils and T cells, and acts as a negative element in basophils and as a positive one in T cells. Thus, this Alu repeat (90% identical to Alu consensus sequences) has evolved to become both a positive and negative regulator.

AB - The FcεRI-γ chains are expressed in a variety of hematopoietic cells where they play a critical role in signal transduction. They are part of the high affinity IgE receptor in mast cells, basophils, Langerhans cells, and possibly other cells; a component of the low affinity receptor for IgG (FcγRIIIA or CD16) in natural killer cells and macrophages; and part of the T cell antigen receptor in subsets of T cells. Here we have investigated the transcriptional regulation of the γ chain gene by analyzing the 2.5-kilobase sequence upstream of the transcription start site. This sequence contains a promoter specific to cells of hematopoietic lineage. However, the tissue specificity of this promoter is only partial because it is active in all of the hematopoietic cells tested here, regardless of whether they constitutively express FcεRI- γ chain transcripts. We have identified two adjacent cis-acting regulatory elements, both of which are part of an Alu repeat. The first (-445/-366) is a positive element active in both basophils and T cells. The second (-365/-264) binds to nuclear factors, which appear to be different in basophils and T cells, and acts as a negative element in basophils and as a positive one in T cells. Thus, this Alu repeat (90% identical to Alu consensus sequences) has evolved to become both a positive and negative regulator.

UR - http://www.scopus.com/inward/record.url?scp=0027465865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027465865&partnerID=8YFLogxK

M3 - Article

C2 - 8419337

AN - SCOPUS:0027465865

VL - 268

SP - 1355

EP - 1361

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 2

ER -