Involvement of an arachidonic-acid-dependent pathway in the interferon-β-mediated expression of C202 gene in Ehrlich-ascites-tumor cells

Elena Toniato, Vincenzo Flati, Maria Grazia Cifone, Egidio Del Grosso, Paola Roncaioli, Lucia Cilenti, Alessandra Tessitore, Florigio Lista, Luigi Frati, Alberto Gulino, Stefano Martinotti

Research output: Contribution to journalArticle

Abstract

We have investigated the signal transduction mechanism of the expression of the C202 gene mediated by interferon β (IFN-β) in the murine Ehrlich's ascites tumor cell line. We have shown that treatment of cells with IFN-β transiently enhances within minutes the release of free arachidonic acid through membrane phospholipase activity. Furthermore, prior treatment with either p-bromophenacyl bromide, an antagonist of both cytosolic and secretory phospholipase A2, or neomycin, which blocks phospholipase C activity, significantly decreased the activation of the murine IFN-β-inducible gene, C202. Moreover, an increase of the expression of the C202 gene was observed after blocking of both the cyclooxygenase and lipoxygenase pathways. This suggests that further metabolism of arachidonic acid to epoxides via epoxygenase-catalysed pathways may be a mechanism by which second messengers for IFN-β-mediated effects on C202 gene expression are generated. Taken together, these results indicate that lipids as second messengers may be important mediators in the IFN-β-based activation of C202 gene expression.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalEuropean Journal of Biochemistry
Volume235
Issue number1-2
Publication statusPublished - 1996

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Keywords

  • arachidonic acid
  • epoxides
  • interferon-β
  • signal transduction

ASJC Scopus subject areas

  • Biochemistry

Cite this

Toniato, E., Flati, V., Cifone, M. G., Del Grosso, E., Roncaioli, P., Cilenti, L., Tessitore, A., Lista, F., Frati, L., Gulino, A., & Martinotti, S. (1996). Involvement of an arachidonic-acid-dependent pathway in the interferon-β-mediated expression of C202 gene in Ehrlich-ascites-tumor cells. European Journal of Biochemistry, 235(1-2), 91-96.