Involvement of bcl-2 and bax gene expression in apoptosis and differentiation of the non-tumorigenic murine hematopoietic cell line, 32DC13(G)

Anna Maria Aglianò, Giuseppe Naso, Ida Silvestri, Angela Gradilone, Roberta Vercillo, Maria Napolitano, Paola Gazzaniga, Orietta Gandini, Massimo Realacci, Carmela Santangelo, Gloria Saccani, Vittorio Manzari, Luigi Frati

Research output: Contribution to journalArticle

Abstract

32DC13(G) is an interleukin-3 (IL-3) dependent, non-tumorigenic murine hematopoietic cell line which undergoes terminal differentiation into granulocytes when exposed to granulocyte-colony stimulating factor (G-CSF). This line therefore offers a convenient system to study the expression of genes involved in apoptosis and differentiation. In our experiments we have acquired evidence that during the differentiation pathway, likewise in apoptosis induced by IL-3 deprivation, detectable levels of bax mRNA appear, while bcl-2 expression decreases. These events are under the control of the p53 tumor-suppressor gene. In these cells, an overexpression of exogenous wild-type p53 leads to a decrease in bcl-2 mRNA and to the appearance of bax mRNA, which instead is absent in the parental cells growing in IL-3 conditioned medium. Furthermore, results from experiments on p53 transfected cells demonstrate that excess wild-type p53 activity, on its own, fails to elicit apoptosis as long as IL-3 is present and does not induce differentiation if G-CSF is not added to the culture medium. We conclude that in apoptosis and differentiation of 32DC13(G) the alterate ratio of bcl-2 and bax gene expression, modulated by p53, is an early event dependent on IL-3 withdrawal and that the appearance of bax and the decrease of bcl-2 expression are necessary, but not sufficient for the acquisition of a completely mature granulocytic phenotype.

Original languageEnglish
Pages (from-to)1271-1277
Number of pages7
JournalInternational Journal of Oncology
Volume11
Issue number6
Publication statusPublished - 1997

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Keywords

  • Apoptosis
  • Bax
  • Bcl-2
  • Differentiation
  • P53
  • Transfection

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Aglianò, A. M., Naso, G., Silvestri, I., Gradilone, A., Vercillo, R., Napolitano, M., Gazzaniga, P., Gandini, O., Realacci, M., Santangelo, C., Saccani, G., Manzari, V., & Frati, L. (1997). Involvement of bcl-2 and bax gene expression in apoptosis and differentiation of the non-tumorigenic murine hematopoietic cell line, 32DC13(G). International Journal of Oncology, 11(6), 1271-1277.