Involvement of HVEM receptor in activation of nuclear factor κB by herpes simplex virus 1 glycoprotein D

Maria Teresa Sciortino, Maria Antonietta Medici, Francesca Marino-Merlo, Daniela Zaccaria, Maria Giuffrè-Cuculletto, Assunta Venuti, Sandro Grelli, Antonio Mastino

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Abstract

The UV-inactivated herpes simplex virus 1 (HSV-1) and glycoprotein D (gD) of HSV-1 have been shown to activate nuclear factor κB (NF-κB) in U937 cells, but mechanisms involved in this activation have not been elucidated. Here we report that: (i) UV-inactivated HSV-1 induced an increased NF-κB activation in cells expressing human HVEM (for herpesvirus entry mediator) at surface level, naturally or following stable transfection, but not in cells in which this receptor was not detected by flow cytometry analysis, (ii) treatment with soluble gD induced a dose-dependent NF-κB activation in THP-1 cells naturally expressing HVEM, and a monoclonal antibody that prevents binding of gD to HVEM significantly reduced NF-κB activation by soluble gD in the same cells, (iii) coculture with transfectants expressing wild-type gD on their surface induced an approximately twofold increase in NF-κB activation in cells naturally expressing HVEM, while coculture with transfectants expressing a mutated form of gD, lacking its capability to bind HVEM, did not induce a similar effect and (iv) treatment with soluble gD induced a dose-dependent NF-κB activation in CHO transfectants expressing HVEM, but not in control CHO transfectants lacking any functional gD receptor. Overall, these results establish that HVEM is involved in NF-κB activation by HSV-1 gD.

Original languageEnglish
Pages (from-to)2297-2311
Number of pages15
JournalCellular Microbiology
Volume10
Issue number11
DOIs
Publication statusPublished - 2008

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Receptors, Tumor Necrosis Factor, Member 14
Glycoproteins
Human Herpesvirus 1
Coculture Techniques
U937 Cells
Human herpesvirus 1 glycoprotein D
Transfection
Flow Cytometry
Monoclonal Antibodies

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Sciortino, M. T., Medici, M. A., Marino-Merlo, F., Zaccaria, D., Giuffrè-Cuculletto, M., Venuti, A., ... Mastino, A. (2008). Involvement of HVEM receptor in activation of nuclear factor κB by herpes simplex virus 1 glycoprotein D. Cellular Microbiology, 10(11), 2297-2311. https://doi.org/10.1111/j.1462-5822.2008.01212.x

Involvement of HVEM receptor in activation of nuclear factor κB by herpes simplex virus 1 glycoprotein D. / Sciortino, Maria Teresa; Medici, Maria Antonietta; Marino-Merlo, Francesca; Zaccaria, Daniela; Giuffrè-Cuculletto, Maria; Venuti, Assunta; Grelli, Sandro; Mastino, Antonio.

In: Cellular Microbiology, Vol. 10, No. 11, 2008, p. 2297-2311.

Research output: Contribution to journalArticle

Sciortino, MT, Medici, MA, Marino-Merlo, F, Zaccaria, D, Giuffrè-Cuculletto, M, Venuti, A, Grelli, S & Mastino, A 2008, 'Involvement of HVEM receptor in activation of nuclear factor κB by herpes simplex virus 1 glycoprotein D', Cellular Microbiology, vol. 10, no. 11, pp. 2297-2311. https://doi.org/10.1111/j.1462-5822.2008.01212.x
Sciortino MT, Medici MA, Marino-Merlo F, Zaccaria D, Giuffrè-Cuculletto M, Venuti A et al. Involvement of HVEM receptor in activation of nuclear factor κB by herpes simplex virus 1 glycoprotein D. Cellular Microbiology. 2008;10(11):2297-2311. https://doi.org/10.1111/j.1462-5822.2008.01212.x
Sciortino, Maria Teresa ; Medici, Maria Antonietta ; Marino-Merlo, Francesca ; Zaccaria, Daniela ; Giuffrè-Cuculletto, Maria ; Venuti, Assunta ; Grelli, Sandro ; Mastino, Antonio. / Involvement of HVEM receptor in activation of nuclear factor κB by herpes simplex virus 1 glycoprotein D. In: Cellular Microbiology. 2008 ; Vol. 10, No. 11. pp. 2297-2311.
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