Involvement of nitric oxide in tumor cell adhesion to cytokine-activated endothelial cells

M. J. Vidal, M. R. Zocchi, A. Poggi, F. Pellegatta, S. L. Chierchia

Research output: Contribution to journalArticle

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Abstract

The aim of the present experiments was to test the possible involvement of nitric oxide (NO) in cytokine-induced enhancement of tumor cell (TC) adhesion to endothelial cells (ECs). Exposure of EA hyb 926 cells to TNF (500 U/ml) plus IFN (100 U/ml) for 24 h significantly enhanced their adhesivity for the 51Cr-labeled GLC1 (small cell lung carcinoma) TCs. Conversely, exposure of TCs to cytokines did not result in an increased adhesion of these cells to ECs. TC-stimulated adhesion to EA hyb 926 was abrogated by the glucocorticoid dexamethasone (Dex, 10-7 M), the NO synthase inhibitors N(ω)-nitro-L- arginine methyl ester (L-NAME, 10-5 M) and N(G)-monomethyl-L-arginine (L- NMMA, 10-5 M) and the protein synthesis inhibitor cyclohexymide (Cex, 10-6 M). Furthermore, GLC1-stimulated adhesion to EA hyb 926 was reversed following removal of L-arginine from the medium or pretreatment with the guanylate cyclase inhibitor methylene blue. TC-stimulated adhesion was also prevented when TCs were pretreated with the monoclonal antibody CD15 directed against the endothelial-leukocyte adhesion molecule (ELAM-1) ligand or following exposure of ECs to anti-ELAM-1 monoclonal antibody. Although suppressing TC-stimulated adhesion, L-NMMA failed to modify significantly cytokine-induced ELAM-1 expression in EA hyb 926. These results (a) provide evidence for the NO-inducible pathway contributing to cytokine-induced enhancement of tumor cell adhesion to the vascular endothelium and (b) demonstrate the involvement of the ELAM-1/CD15 adhesion system in tumor cell- stimulated adhesion to ECs.

Original languageEnglish
JournalJournal of Cardiovascular Pharmacology
Volume20
Issue numberSUPPL. 12
Publication statusPublished - 1992

Fingerprint

Cell Adhesion
E-Selectin
Nitric Oxide
Endothelial Cells
Cytokines
Neoplasms
omega-N-Methylarginine
Arginine
Monoclonal Antibodies
Protein Synthesis Inhibitors
Guanylate Cyclase
Methylene Blue
NG-Nitroarginine Methyl Ester
Small Cell Lung Carcinoma
Vascular Endothelium
Nitric Oxide Synthase
Dexamethasone
Glucocorticoids
Ligands

Keywords

  • Adhesion
  • Cytokines
  • Endothelial cells
  • Nitric oxide
  • Tumor cells

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pharmacology

Cite this

Involvement of nitric oxide in tumor cell adhesion to cytokine-activated endothelial cells. / Vidal, M. J.; Zocchi, M. R.; Poggi, A.; Pellegatta, F.; Chierchia, S. L.

In: Journal of Cardiovascular Pharmacology, Vol. 20, No. SUPPL. 12, 1992.

Research output: Contribution to journalArticle

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AU - Chierchia, S. L.

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