Involvement of PGC-1, NRF-1, and NRF-2 in metabolic response by rat liver to hormonal and environmental signals

P. Venditti, A. Bari, L. Di Stefano, A. Cardone, F. Della Ragione, M. D'Esposito, S. Di Meo

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We studied liver oxidative capacity and O2 consumption in hypothyroid rats treated for 10 days with T4, or T3, or treated for 10 days with T3 and exposed to cold for the last 2 days. The metabolic response of homogenates and mitochondria indicated that all treatments increased the synthesis of respiratory chain components, whereas only the cold-induced mitochondrial proliferation. Determination of mRNA and protein expression of transcription factor activators, such as NRF-1 and NRF-2, and coactivators, such as PGC-1, showed that mRNA levels, except PGC-1 ones, were not related to aerobic capacities. Conversely, a strong correlation was found between cytochrome oxidase activity and PGC-1 or NRF-2 protein levels. Such a correlation was not found for NRF-1. Our results strongly support the view that in rat liver PGC-1 and NRFs are responsible for the iodothyronine-induced increases in respiratory chain components, whereas their role in cold-induced mitochondrial proliferation needs to be further on clarified.

Original languageEnglish
Pages (from-to)22-29
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume305
Issue number1-2
DOIs
Publication statusPublished - Jun 16 2009

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Liver
Rats
Electron Transport
Messenger RNA
Mitochondria
Electron Transport Complex IV
Proteins
Transcription Factors

Keywords

  • Cold exposure
  • Mitochondrial proliferation
  • Mitochondrial respiration
  • Thyroid hormone
  • Transcription factor activators

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Involvement of PGC-1, NRF-1, and NRF-2 in metabolic response by rat liver to hormonal and environmental signals. / Venditti, P.; Bari, A.; Di Stefano, L.; Cardone, A.; Della Ragione, F.; D'Esposito, M.; Di Meo, S.

In: Molecular and Cellular Endocrinology, Vol. 305, No. 1-2, 16.06.2009, p. 22-29.

Research output: Contribution to journalArticle

Venditti, P. ; Bari, A. ; Di Stefano, L. ; Cardone, A. ; Della Ragione, F. ; D'Esposito, M. ; Di Meo, S. / Involvement of PGC-1, NRF-1, and NRF-2 in metabolic response by rat liver to hormonal and environmental signals. In: Molecular and Cellular Endocrinology. 2009 ; Vol. 305, No. 1-2. pp. 22-29.
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