Involvement of phospholipase Cγ1 in mouse egg activation induced by a truncated form of the C-kit tyrosine kinase present in spermatozoa

Claudio Sette, Arturo Bevilacqua, Raffaele Geremia, Pellegrino Rossi

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Microinjection of a truncated form of the c-kit tyrosine kinase present in mouse spermatozoa (tr-kit) activates mouse eggs parthenogenetically, and tr-kit-induced egg activation is inhibited by preincubation with an inhibitor of phospholipase C (PLC) (Sette, C., A. Bevilacqua, A. Bianchini, F. Mangia, R. Geremia, and P. Rossi. 1997. Development [Camb.]. 124:2267-2274). Coinjection of glutathione-S-transferase (GST) fusion proteins containing the src-homology (SH) domains of the γ1 isoform of PLC (PLCγ1) competitively inhibits tr-kit-induced egg activation. A GST fusion protein containing the SH3 domain of PLCγ1 inhibits egg activation as efficiently as the whole SH region, while a GST fusion protein containing the two SH2 domains is much less effective. A GST fusion protein containing the SH3 domain of the Grb2 adaptor protein does not inhibit tr-kit-induced egg activation, showing that the effect of the SH3 domain of PLCγ1 is specific. Tr-kit-induced egg activation is also suppressed by co-injection of antibodies raised against the PLCγ1 SH domains, but not against the PLCγ1 COOH-terminal region. In transfected COS cells, coexpression of PLCγ1 and tr-kit increases diacylglycerol and inositol phosphate production, and the phosphotyrosine content of PLCγ1 with respect to cells expressing PLCγ1 alone. These data indicate that tr-kit activates PLCγ1, and that the SH3 domain of PLCγ1 is essential for tr-kit-induced egg activation.

Original languageEnglish
Pages (from-to)1063-1074
Number of pages12
JournalJournal of Cell Biology
Volume142
Issue number4
DOIs
Publication statusPublished - Aug 24 1998

Fingerprint

src Homology Domains
Protein-Tyrosine Kinases
Ovum
Spermatozoa
Glutathione Transferase
Type C Phospholipases
Proteins
Phosphotyrosine
Inositol Phosphates
COS Cells
Diglycerides
Microinjections
phospholipase C1
Eggs
Protein Isoforms
Injections
Antibodies

Keywords

  • Egg-activation
  • Phospholipase-C-γ1
  • Spermatozoa
  • Src- homology-domains
  • Truncated-c-kit

ASJC Scopus subject areas

  • Cell Biology

Cite this

Involvement of phospholipase Cγ1 in mouse egg activation induced by a truncated form of the C-kit tyrosine kinase present in spermatozoa. / Sette, Claudio; Bevilacqua, Arturo; Geremia, Raffaele; Rossi, Pellegrino.

In: Journal of Cell Biology, Vol. 142, No. 4, 24.08.1998, p. 1063-1074.

Research output: Contribution to journalArticle

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