Involvement of the potassium-dependent sodium/calcium exchanger gene product NCKX2 in the brain insult induced by permanent focal cerebral ischemia

Ornella Cuomo, Giuseppe Pignataro, Rosaria Gala, Francesca Boscia, Anna Tortiglione, Pasquale Molinaro, Gianfranco Di Renzo, Jonathan Lytton, Lucio Annunziato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Sodium/calcium exchangers are neuronal plasma membrane transporters, which by coupling Ca2+ and Na+ fluxes, may play a relevant role in brain ischemia. The exchanger gene superfamily comprises two arms: the K +-independent (NCX) and K+-dependent (NCKX) exchangers. In the brain, three different NCX (NCX1, NCX2, NCX3) and three NCKX (NCKX2, NCKX3, NCKX4) family members have been described. Up to now, no sutides about the role played by NCKX proteins in cerebral ischemia have been published. The aim of the present study was to investigate the role of NCKX2 in an in vivo model of permanent middle cerebral artery occlusion (pMCAO). The role of this protein in the development of ischemic damage was assessed by knocking-down its expression with an antisense oligodeoxynucleotide (AS-ODN), intra-cerebroventricularly infused by an osmotic minipump for 48 h, starting from 24 h before pMCAO. The results showed that NCKX2 knocking-down by using antisense strategy increased the extent of the ischemic lesion. The results of this study suggest that NCKX2 could exert a neuroprotective effect during ischemic injury.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages486-489
Number of pages4
Volume1099
DOIs
Publication statusPublished - Mar 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1099
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • Antisense oligodeoxynucleotides
  • Cerebral ischemia
  • Na/Ca exchanger
  • NCKX2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Involvement of the potassium-dependent sodium/calcium exchanger gene product NCKX2 in the brain insult induced by permanent focal cerebral ischemia'. Together they form a unique fingerprint.

  • Cite this

    Cuomo, O., Pignataro, G., Gala, R., Boscia, F., Tortiglione, A., Molinaro, P., Di Renzo, G., Lytton, J., & Annunziato, L. (2007). Involvement of the potassium-dependent sodium/calcium exchanger gene product NCKX2 in the brain insult induced by permanent focal cerebral ischemia. In Annals of the New York Academy of Sciences (Vol. 1099, pp. 486-489). (Annals of the New York Academy of Sciences; Vol. 1099). https://doi.org/10.1196/annals.1387.051