Ion trap liquid chromatography/tandem mass spectrometry analysis of leukotriene B4 in exhaled breath condensate

Paolo Montuschi, Simona Martello, Marialinda Felli, Chiara Mondino, Marcello Chiarotti

Research output: Contribution to journalArticle

Abstract

The objective of this study is the measurement of leukotriene B4 (LTB4), a potent inflammatory mediator, in exhaled breath condensate by using liquid chromatography/mass spectrometry (LC/MS and LC/MS/MS). Condensation of exhaled breath is a non-invasive method to collect airway secretions. Deuterated (d4)-LTB4 was used as internal standard. The MS and MS/MS behavior of LTB4 and LTB 4-d4 was studied by electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) in both positive and negative ion polarity mode. Preliminary results show that monitoring negative ions in ESI mode has the best sensitivity for both LTB4 and LTB 4-d4. Therefore, negative ESI was chosen, and the [M-H]- ions at m/z 335 and 339 were selected for quantification. The lower limit of quantification for LTB4, expressed as the lowest point of the calibration curve, was 100 pg/mL. Using this technique, we measured LTB4 in exhaled breath condensate in two healthy subjects, four asthmatic patients on anti-inflammatory treatment, and four asthmatic patients who were not on anti-inflammatory drugs. Exhaled LTB4 concentrations were detected only in asthmatic patients who were not on anti-inflammatory therapy. This method is potentially useful for non-invasive assessment of airway inflammation, but the sensitivity of the technique needs to be improved.

Original languageEnglish
Pages (from-to)2723-2729
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume18
Issue number22
DOIs
Publication statusPublished - 2004

Fingerprint

Leukotriene B4
Liquid chromatography
Mass spectrometry
Ions
Electrospray ionization
Anti-Inflammatory Agents
Negative ions
Atmospheric pressure
Ionization
Condensation
Positive ions
Calibration
Monitoring
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Ion trap liquid chromatography/tandem mass spectrometry analysis of leukotriene B4 in exhaled breath condensate. / Montuschi, Paolo; Martello, Simona; Felli, Marialinda; Mondino, Chiara; Chiarotti, Marcello.

In: Rapid Communications in Mass Spectrometry, Vol. 18, No. 22, 2004, p. 2723-2729.

Research output: Contribution to journalArticle

Montuschi, Paolo ; Martello, Simona ; Felli, Marialinda ; Mondino, Chiara ; Chiarotti, Marcello. / Ion trap liquid chromatography/tandem mass spectrometry analysis of leukotriene B4 in exhaled breath condensate. In: Rapid Communications in Mass Spectrometry. 2004 ; Vol. 18, No. 22. pp. 2723-2729.
@article{a34c37a4b97943089c4412776845fe04,
title = "Ion trap liquid chromatography/tandem mass spectrometry analysis of leukotriene B4 in exhaled breath condensate",
abstract = "The objective of this study is the measurement of leukotriene B4 (LTB4), a potent inflammatory mediator, in exhaled breath condensate by using liquid chromatography/mass spectrometry (LC/MS and LC/MS/MS). Condensation of exhaled breath is a non-invasive method to collect airway secretions. Deuterated (d4)-LTB4 was used as internal standard. The MS and MS/MS behavior of LTB4 and LTB 4-d4 was studied by electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) in both positive and negative ion polarity mode. Preliminary results show that monitoring negative ions in ESI mode has the best sensitivity for both LTB4 and LTB 4-d4. Therefore, negative ESI was chosen, and the [M-H]- ions at m/z 335 and 339 were selected for quantification. The lower limit of quantification for LTB4, expressed as the lowest point of the calibration curve, was 100 pg/mL. Using this technique, we measured LTB4 in exhaled breath condensate in two healthy subjects, four asthmatic patients on anti-inflammatory treatment, and four asthmatic patients who were not on anti-inflammatory drugs. Exhaled LTB4 concentrations were detected only in asthmatic patients who were not on anti-inflammatory therapy. This method is potentially useful for non-invasive assessment of airway inflammation, but the sensitivity of the technique needs to be improved.",
author = "Paolo Montuschi and Simona Martello and Marialinda Felli and Chiara Mondino and Marcello Chiarotti",
year = "2004",
doi = "10.1002/rcm.1682",
language = "English",
volume = "18",
pages = "2723--2729",
journal = "Rapid Communications in Mass Spectrometry",
issn = "0951-4198",
publisher = "John Wiley and Sons Ltd",
number = "22",

}

TY - JOUR

T1 - Ion trap liquid chromatography/tandem mass spectrometry analysis of leukotriene B4 in exhaled breath condensate

AU - Montuschi, Paolo

AU - Martello, Simona

AU - Felli, Marialinda

AU - Mondino, Chiara

AU - Chiarotti, Marcello

PY - 2004

Y1 - 2004

N2 - The objective of this study is the measurement of leukotriene B4 (LTB4), a potent inflammatory mediator, in exhaled breath condensate by using liquid chromatography/mass spectrometry (LC/MS and LC/MS/MS). Condensation of exhaled breath is a non-invasive method to collect airway secretions. Deuterated (d4)-LTB4 was used as internal standard. The MS and MS/MS behavior of LTB4 and LTB 4-d4 was studied by electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) in both positive and negative ion polarity mode. Preliminary results show that monitoring negative ions in ESI mode has the best sensitivity for both LTB4 and LTB 4-d4. Therefore, negative ESI was chosen, and the [M-H]- ions at m/z 335 and 339 were selected for quantification. The lower limit of quantification for LTB4, expressed as the lowest point of the calibration curve, was 100 pg/mL. Using this technique, we measured LTB4 in exhaled breath condensate in two healthy subjects, four asthmatic patients on anti-inflammatory treatment, and four asthmatic patients who were not on anti-inflammatory drugs. Exhaled LTB4 concentrations were detected only in asthmatic patients who were not on anti-inflammatory therapy. This method is potentially useful for non-invasive assessment of airway inflammation, but the sensitivity of the technique needs to be improved.

AB - The objective of this study is the measurement of leukotriene B4 (LTB4), a potent inflammatory mediator, in exhaled breath condensate by using liquid chromatography/mass spectrometry (LC/MS and LC/MS/MS). Condensation of exhaled breath is a non-invasive method to collect airway secretions. Deuterated (d4)-LTB4 was used as internal standard. The MS and MS/MS behavior of LTB4 and LTB 4-d4 was studied by electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) in both positive and negative ion polarity mode. Preliminary results show that monitoring negative ions in ESI mode has the best sensitivity for both LTB4 and LTB 4-d4. Therefore, negative ESI was chosen, and the [M-H]- ions at m/z 335 and 339 were selected for quantification. The lower limit of quantification for LTB4, expressed as the lowest point of the calibration curve, was 100 pg/mL. Using this technique, we measured LTB4 in exhaled breath condensate in two healthy subjects, four asthmatic patients on anti-inflammatory treatment, and four asthmatic patients who were not on anti-inflammatory drugs. Exhaled LTB4 concentrations were detected only in asthmatic patients who were not on anti-inflammatory therapy. This method is potentially useful for non-invasive assessment of airway inflammation, but the sensitivity of the technique needs to be improved.

UR - http://www.scopus.com/inward/record.url?scp=9144272726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9144272726&partnerID=8YFLogxK

U2 - 10.1002/rcm.1682

DO - 10.1002/rcm.1682

M3 - Article

C2 - 15499663

AN - SCOPUS:9144272726

VL - 18

SP - 2723

EP - 2729

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 22

ER -