Ionic regulation of human basophil releasability. I. Inhibitory effect of copper

A. Tedeschi, M. Arquati, M. Lorini, N. Milazzo, A. Miadonna

Research output: Contribution to journalArticle

Abstract

The effects of copper (CuSO4 and CuCl2) on in vitro histamine release from human basophils stimulated by anti-IgE and Ca2+ ionophore A23187 were evaluated. Both CuSO4 and CuCl2 caused a dose-related inhibition of histamine release, which was more pronounced on anti-IgE-than on Ca2+ ionophore-induced histamine release. The concentration which produced 50% inhibition of anti-IgE-induced histamine release was 1.3 μM for CuSO4 and 1.5 μM for CuCl2; the maximal inhibition of Ca2+ ionophore-induced histamine release was 33% for CuCl2 (4 μM) and 51% for CuSO4 (16 μM). The inhibitory effect on anti-IgE-induced histamine release persisted also when extracellular Cu2+ was removed by cell washing before IgE-induced histamine release persisted also when extracellular Cu2+ was removed by cell washing before stimulation, whereas no inhibition of Ca2+ ionophore-induced histamine release was found when extracellular Cu2+ was removed. The activity of Cu2+ was independent of any effects of deuterium oxide and colchicine, two agents known to interact with microtubules. Increased extracellular Ca2+ concentrations reduced the inhibitory effect of CuCl2 on Ca2+ ionophore-induced histamine release, and Schild plot analysis demonstrated that Cu2+ ions are competitive antagonists of Ca2+ ions. These results indicate that Cu2+ ions in the micromolar range down-regulate anti-IgE- and Ca2+ ionophore-induced histamine release. Since Cu2+ concentration in human plasma is in the micromolar range (30 μM with 10-30% of free Cu2+), it is conceivable that Cu2+ ions contribute to the in vivo regulation of histamine release from human basophils.

Original languageEnglish
Pages (from-to)16-24
Number of pages9
JournalAgents and Actions
Volume37
Issue number1-2
DOIs
Publication statusPublished - Sep 1992

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)

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    Tedeschi, A., Arquati, M., Lorini, M., Milazzo, N., & Miadonna, A. (1992). Ionic regulation of human basophil releasability. I. Inhibitory effect of copper. Agents and Actions, 37(1-2), 16-24. https://doi.org/10.1007/BF01987885