Abstract
Objective: In Indian HIV-infected patients, IP-10 response to QuantiFERON-TB Gold In tube (QFT-IT) antigens has been associated to tuberculosis (TB). However, specificity for active TB was lower than that reported by QFT-IT, making accuracy for TB detection questionable. To investigate this uncertainty, likely due to India being highly endemic for TB, and to better identify TB correlates, we evaluated the IP-10-based assay in HIV-infected subjects in Italy, a low-TB endemic country. Methods: 195 individuals were prospectively enrolled; 118 were HIV-infected (21 with active TB, 97 without active TB, and distinguished as high/low-TB-risk). QFT-IT was performed and IP-10 was evaluated by ELISA. Results: Among the HIV-infected individuals, sensitivity for active TB was 66.7% by IP-10-based test and 52.4% (p = 1) by QFT-IT. IP-10-based assay showed a lower dependence on mitogen-response and CD4 counts than QFT-IT. Among subjects without active TB, a higher proportion of IP-10 responders was shown in high-TB-risk subjects than low-TB-risk subjects (40.0% vs 12.9%), similar to QFT-IT (37.1% vs 4.8%). Low-TB risk subjects showed 87.1% specificity for active TB by IP-10-based test vs 95.2% by QFT-IT. Conclusions: In a low-TB endemic country, besides IFN-γ, IP-10 response to QFT-IT is associated with active TB and TB risk factors in HIV-infected patients with lower dependence on mitogen-response and CD4 counts.
| Original language | English |
|---|---|
| Pages (from-to) | 49-59 |
| Number of pages | 11 |
| Journal | Journal of Infection |
| Volume | 65 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 2012 |
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Keywords
- Diagnosis
- HIV
- IGRA
- Immune deficiency
- IP-10
- Low TB endemic country
- QuantiFERON
- Tuberculosis
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases
Cite this
IP-10 is an additional marker for tuberculosis (TB) detection in HIV-infected persons in a low-TB endemic country. / Vanini, Valentina; Petruccioli, Elisa; Gioia, Cristiana; Cuzzi, Gilda; Orchi, Nicoletta; Rianda, Alessia; Alba, Lucia; Giancola, Maria Letizia; Conte, Aristide; Schininà, Vincenzo; Busi Rizzi, Elisa; Girardi, Enrico; Goletti, Delia.
In: Journal of Infection, Vol. 65, No. 1, 07.2012, p. 49-59.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - IP-10 is an additional marker for tuberculosis (TB) detection in HIV-infected persons in a low-TB endemic country
AU - Vanini, Valentina
AU - Petruccioli, Elisa
AU - Gioia, Cristiana
AU - Cuzzi, Gilda
AU - Orchi, Nicoletta
AU - Rianda, Alessia
AU - Alba, Lucia
AU - Giancola, Maria Letizia
AU - Conte, Aristide
AU - Schininà, Vincenzo
AU - Busi Rizzi, Elisa
AU - Girardi, Enrico
AU - Goletti, Delia
PY - 2012/7
Y1 - 2012/7
N2 - Objective: In Indian HIV-infected patients, IP-10 response to QuantiFERON-TB Gold In tube (QFT-IT) antigens has been associated to tuberculosis (TB). However, specificity for active TB was lower than that reported by QFT-IT, making accuracy for TB detection questionable. To investigate this uncertainty, likely due to India being highly endemic for TB, and to better identify TB correlates, we evaluated the IP-10-based assay in HIV-infected subjects in Italy, a low-TB endemic country. Methods: 195 individuals were prospectively enrolled; 118 were HIV-infected (21 with active TB, 97 without active TB, and distinguished as high/low-TB-risk). QFT-IT was performed and IP-10 was evaluated by ELISA. Results: Among the HIV-infected individuals, sensitivity for active TB was 66.7% by IP-10-based test and 52.4% (p = 1) by QFT-IT. IP-10-based assay showed a lower dependence on mitogen-response and CD4 counts than QFT-IT. Among subjects without active TB, a higher proportion of IP-10 responders was shown in high-TB-risk subjects than low-TB-risk subjects (40.0% vs 12.9%), similar to QFT-IT (37.1% vs 4.8%). Low-TB risk subjects showed 87.1% specificity for active TB by IP-10-based test vs 95.2% by QFT-IT. Conclusions: In a low-TB endemic country, besides IFN-γ, IP-10 response to QFT-IT is associated with active TB and TB risk factors in HIV-infected patients with lower dependence on mitogen-response and CD4 counts.
AB - Objective: In Indian HIV-infected patients, IP-10 response to QuantiFERON-TB Gold In tube (QFT-IT) antigens has been associated to tuberculosis (TB). However, specificity for active TB was lower than that reported by QFT-IT, making accuracy for TB detection questionable. To investigate this uncertainty, likely due to India being highly endemic for TB, and to better identify TB correlates, we evaluated the IP-10-based assay in HIV-infected subjects in Italy, a low-TB endemic country. Methods: 195 individuals were prospectively enrolled; 118 were HIV-infected (21 with active TB, 97 without active TB, and distinguished as high/low-TB-risk). QFT-IT was performed and IP-10 was evaluated by ELISA. Results: Among the HIV-infected individuals, sensitivity for active TB was 66.7% by IP-10-based test and 52.4% (p = 1) by QFT-IT. IP-10-based assay showed a lower dependence on mitogen-response and CD4 counts than QFT-IT. Among subjects without active TB, a higher proportion of IP-10 responders was shown in high-TB-risk subjects than low-TB-risk subjects (40.0% vs 12.9%), similar to QFT-IT (37.1% vs 4.8%). Low-TB risk subjects showed 87.1% specificity for active TB by IP-10-based test vs 95.2% by QFT-IT. Conclusions: In a low-TB endemic country, besides IFN-γ, IP-10 response to QFT-IT is associated with active TB and TB risk factors in HIV-infected patients with lower dependence on mitogen-response and CD4 counts.
KW - Diagnosis
KW - HIV
KW - IGRA
KW - Immune deficiency
KW - IP-10
KW - Low TB endemic country
KW - QuantiFERON
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=84861481668&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861481668&partnerID=8YFLogxK
U2 - 10.1016/j.jinf.2012.03.017
DO - 10.1016/j.jinf.2012.03.017
M3 - Article
C2 - 22465752
AN - SCOPUS:84861481668
VL - 65
SP - 49
EP - 59
JO - Journal of Infection
JF - Journal of Infection
SN - 0163-4453
IS - 1
ER -