IP-10 is an additional marker for tuberculosis (TB) detection in HIV-infected persons in a low-TB endemic country

Research output: Contribution to journalArticle

Abstract

Objective: In Indian HIV-infected patients, IP-10 response to QuantiFERON-TB Gold In tube (QFT-IT) antigens has been associated to tuberculosis (TB). However, specificity for active TB was lower than that reported by QFT-IT, making accuracy for TB detection questionable. To investigate this uncertainty, likely due to India being highly endemic for TB, and to better identify TB correlates, we evaluated the IP-10-based assay in HIV-infected subjects in Italy, a low-TB endemic country. Methods: 195 individuals were prospectively enrolled; 118 were HIV-infected (21 with active TB, 97 without active TB, and distinguished as high/low-TB-risk). QFT-IT was performed and IP-10 was evaluated by ELISA. Results: Among the HIV-infected individuals, sensitivity for active TB was 66.7% by IP-10-based test and 52.4% (p = 1) by QFT-IT. IP-10-based assay showed a lower dependence on mitogen-response and CD4 counts than QFT-IT. Among subjects without active TB, a higher proportion of IP-10 responders was shown in high-TB-risk subjects than low-TB-risk subjects (40.0% vs 12.9%), similar to QFT-IT (37.1% vs 4.8%). Low-TB risk subjects showed 87.1% specificity for active TB by IP-10-based test vs 95.2% by QFT-IT. Conclusions: In a low-TB endemic country, besides IFN-γ, IP-10 response to QFT-IT is associated with active TB and TB risk factors in HIV-infected patients with lower dependence on mitogen-response and CD4 counts.

Original languageEnglish
Pages (from-to)49-59
Number of pages11
JournalJournal of Infection
Volume65
Issue number1
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Tuberculosis
HIV
Gold
CD4 Lymphocyte Count
Mitogens
Italy
Uncertainty
India

Keywords

  • Diagnosis
  • HIV
  • IGRA
  • Immune deficiency
  • IP-10
  • Low TB endemic country
  • QuantiFERON
  • Tuberculosis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

@article{397d220d243141ccbb3f36b9632ffff4,
title = "IP-10 is an additional marker for tuberculosis (TB) detection in HIV-infected persons in a low-TB endemic country",
abstract = "Objective: In Indian HIV-infected patients, IP-10 response to QuantiFERON-TB Gold In tube (QFT-IT) antigens has been associated to tuberculosis (TB). However, specificity for active TB was lower than that reported by QFT-IT, making accuracy for TB detection questionable. To investigate this uncertainty, likely due to India being highly endemic for TB, and to better identify TB correlates, we evaluated the IP-10-based assay in HIV-infected subjects in Italy, a low-TB endemic country. Methods: 195 individuals were prospectively enrolled; 118 were HIV-infected (21 with active TB, 97 without active TB, and distinguished as high/low-TB-risk). QFT-IT was performed and IP-10 was evaluated by ELISA. Results: Among the HIV-infected individuals, sensitivity for active TB was 66.7{\%} by IP-10-based test and 52.4{\%} (p = 1) by QFT-IT. IP-10-based assay showed a lower dependence on mitogen-response and CD4 counts than QFT-IT. Among subjects without active TB, a higher proportion of IP-10 responders was shown in high-TB-risk subjects than low-TB-risk subjects (40.0{\%} vs 12.9{\%}), similar to QFT-IT (37.1{\%} vs 4.8{\%}). Low-TB risk subjects showed 87.1{\%} specificity for active TB by IP-10-based test vs 95.2{\%} by QFT-IT. Conclusions: In a low-TB endemic country, besides IFN-γ, IP-10 response to QFT-IT is associated with active TB and TB risk factors in HIV-infected patients with lower dependence on mitogen-response and CD4 counts.",
keywords = "Diagnosis, HIV, IGRA, Immune deficiency, IP-10, Low TB endemic country, QuantiFERON, Tuberculosis",
author = "Valentina Vanini and Elisa Petruccioli and Cristiana Gioia and Gilda Cuzzi and Nicoletta Orchi and Alessia Rianda and Lucia Alba and Giancola, {Maria Letizia} and Aristide Conte and Vincenzo Schinin{\`a} and {Busi Rizzi}, Elisa and Enrico Girardi and Delia Goletti",
year = "2012",
month = "7",
doi = "10.1016/j.jinf.2012.03.017",
language = "English",
volume = "65",
pages = "49--59",
journal = "Journal of Infection",
issn = "0163-4453",
publisher = "W.B. Saunders Ltd",
number = "1",

}

TY - JOUR

T1 - IP-10 is an additional marker for tuberculosis (TB) detection in HIV-infected persons in a low-TB endemic country

AU - Vanini, Valentina

AU - Petruccioli, Elisa

AU - Gioia, Cristiana

AU - Cuzzi, Gilda

AU - Orchi, Nicoletta

AU - Rianda, Alessia

AU - Alba, Lucia

AU - Giancola, Maria Letizia

AU - Conte, Aristide

AU - Schininà, Vincenzo

AU - Busi Rizzi, Elisa

AU - Girardi, Enrico

AU - Goletti, Delia

PY - 2012/7

Y1 - 2012/7

N2 - Objective: In Indian HIV-infected patients, IP-10 response to QuantiFERON-TB Gold In tube (QFT-IT) antigens has been associated to tuberculosis (TB). However, specificity for active TB was lower than that reported by QFT-IT, making accuracy for TB detection questionable. To investigate this uncertainty, likely due to India being highly endemic for TB, and to better identify TB correlates, we evaluated the IP-10-based assay in HIV-infected subjects in Italy, a low-TB endemic country. Methods: 195 individuals were prospectively enrolled; 118 were HIV-infected (21 with active TB, 97 without active TB, and distinguished as high/low-TB-risk). QFT-IT was performed and IP-10 was evaluated by ELISA. Results: Among the HIV-infected individuals, sensitivity for active TB was 66.7% by IP-10-based test and 52.4% (p = 1) by QFT-IT. IP-10-based assay showed a lower dependence on mitogen-response and CD4 counts than QFT-IT. Among subjects without active TB, a higher proportion of IP-10 responders was shown in high-TB-risk subjects than low-TB-risk subjects (40.0% vs 12.9%), similar to QFT-IT (37.1% vs 4.8%). Low-TB risk subjects showed 87.1% specificity for active TB by IP-10-based test vs 95.2% by QFT-IT. Conclusions: In a low-TB endemic country, besides IFN-γ, IP-10 response to QFT-IT is associated with active TB and TB risk factors in HIV-infected patients with lower dependence on mitogen-response and CD4 counts.

AB - Objective: In Indian HIV-infected patients, IP-10 response to QuantiFERON-TB Gold In tube (QFT-IT) antigens has been associated to tuberculosis (TB). However, specificity for active TB was lower than that reported by QFT-IT, making accuracy for TB detection questionable. To investigate this uncertainty, likely due to India being highly endemic for TB, and to better identify TB correlates, we evaluated the IP-10-based assay in HIV-infected subjects in Italy, a low-TB endemic country. Methods: 195 individuals were prospectively enrolled; 118 were HIV-infected (21 with active TB, 97 without active TB, and distinguished as high/low-TB-risk). QFT-IT was performed and IP-10 was evaluated by ELISA. Results: Among the HIV-infected individuals, sensitivity for active TB was 66.7% by IP-10-based test and 52.4% (p = 1) by QFT-IT. IP-10-based assay showed a lower dependence on mitogen-response and CD4 counts than QFT-IT. Among subjects without active TB, a higher proportion of IP-10 responders was shown in high-TB-risk subjects than low-TB-risk subjects (40.0% vs 12.9%), similar to QFT-IT (37.1% vs 4.8%). Low-TB risk subjects showed 87.1% specificity for active TB by IP-10-based test vs 95.2% by QFT-IT. Conclusions: In a low-TB endemic country, besides IFN-γ, IP-10 response to QFT-IT is associated with active TB and TB risk factors in HIV-infected patients with lower dependence on mitogen-response and CD4 counts.

KW - Diagnosis

KW - HIV

KW - IGRA

KW - Immune deficiency

KW - IP-10

KW - Low TB endemic country

KW - QuantiFERON

KW - Tuberculosis

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U2 - 10.1016/j.jinf.2012.03.017

DO - 10.1016/j.jinf.2012.03.017

M3 - Article

C2 - 22465752

AN - SCOPUS:84861481668

VL - 65

SP - 49

EP - 59

JO - Journal of Infection

JF - Journal of Infection

SN - 0163-4453

IS - 1

ER -