Iron detoxifying activity of ferritin. Effects of H and L human apoferritins on lipid peroxidation in vitro

Research output: Contribution to journalArticle

Abstract

Three recombinant human apoferritin variants were added to ferrous iron and the amount of lipid peroxidation produced by hydrogen peroxide was studied. The H-apoferritin had the strongest inhibitory effect on lipid peroxidation, probably due to its ferroxidase activity. The L-apoferritin inhibited lipid peroxidation slowly and only at neutral pH. The H-mutant 91, deleted of the last 22 C-terminal amino acids, and which is not able to form an iron core, had minimal effects on iron lipid peroxidation. It was concluded that both ferro-oxidase and iron mineralization activities are necessary for ferritin iron detoxifying action.

Original languageEnglish
Pages (from-to)119-122
Number of pages4
JournalFEBS Letters
Volume277
Issue number1-2
DOIs
Publication statusPublished - Dec 17 1990

Keywords

  • Iron toxicity
  • Lipid peroxidation
  • Mutant protein
  • Recombinant ferritin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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