Abstract
The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways. Copyright (C) 2000 Federation of European Biochemical Societies.
Original language | English |
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Pages (from-to) | 208-212 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 469 |
Issue number | 2-3 |
DOIs | |
Publication status | Published - Mar 10 2000 |
Keywords
- Aldose reductase
- Antioxidant
- Apolipoprotein B
- Differential display
- Hepatocyte
- Semaphorin cd100
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology