Iron overload and gene expression in HepG2 cells: Analysis by differential display

Donatella Barisani, Raffaella Meneveri, Enrico Ginelli, Camilla Cassani, Dario Conte

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)208-212
Number of pages5
JournalFEBS Letters
Volume469
Issue number2-3
DOIs
Publication statusPublished - Mar 10 2000

Keywords

  • Aldose reductase
  • Antioxidant
  • Apolipoprotein B
  • Differential display
  • Hepatocyte
  • Semaphorin cd100

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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