Iron overload and gene expression in HepG2 cells: Analysis by differential display

Donatella Barisani; Raffaella Meneveri; Enrico Ginelli; Camilla Cassani; Dario Conte

doi:10.1016/S0014-5793(00)01280-1

Iron overload and gene expression in HepG2 cells: Analysis by differential display

Donatella Barisani, Raffaella Meneveri, Enrico Ginelli, Camilla Cassani, Dario Conte

Fondazione IRCCS Ca' Granda- Ospedale Maggiore Policlinico

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways. Copyright (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	208-212
Number of pages	5
Journal	FEBS Letters
Volume	469
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(00)01280-1
Publication status	Published - Mar 10 2000

Keywords

Aldose reductase
Antioxidant
Apolipoprotein B
Differential display
Hepatocyte
Semaphorin cd100

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/S0014-5793(00)01280-1

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title = "Iron overload and gene expression in HepG2 cells: Analysis by differential display",

abstract = "The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways. Copyright (C) 2000 Federation of European Biochemical Societies.",

keywords = "Aldose reductase, Antioxidant, Apolipoprotein B, Differential display, Hepatocyte, Semaphorin cd100",

author = "Donatella Barisani and Raffaella Meneveri and Enrico Ginelli and Camilla Cassani and Dario Conte",

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T2 - Analysis by differential display

AU - Barisani, Donatella

AU - Meneveri, Raffaella

AU - Ginelli, Enrico

AU - Cassani, Camilla

AU - Conte, Dario

PY - 2000/3/10

Y1 - 2000/3/10

N2 - The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways. Copyright (C) 2000 Federation of European Biochemical Societies.

AB - The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways. Copyright (C) 2000 Federation of European Biochemical Societies.

KW - Aldose reductase

KW - Antioxidant

KW - Apolipoprotein B

KW - Differential display

KW - Hepatocyte

KW - Semaphorin cd100

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Iron overload and gene expression in HepG2 cells: Analysis by differential display

Abstract

Keywords

ASJC Scopus subject areas

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