Iron release and oxidant damage in human myoblasts by divicine

Paolino Ninfali, Maria Paola Perini, Nereo Bresolin, Gianfranca Aluigi, Caterina Cambiaggi, Marco Ferrali, Alfonso Pompella

Research output: Contribution to journalArticle

Abstract

Divicine is an aglycone derived from vicine, a glucosidic compound contained in fava beans (Vicia faba major or broad beans). In this study, we investigated the effect of divicine on cultured human myoblasts from normal subjects, in order to see if the drug may induce signs of oxidant stress in these cells. Myoblasts incubated 24 hours in the presence of 1 mM divicine, showed an increase of carbonyl groups and 4-hydroxynonenal (4-HNE) bound to cell proteins, as well as a significant release of iron and lactate dehydrogenase in the culture medium. Desferrioxamine (DFO), an iron chelator, significantly prevented protein oxidation and formation 4-HNE adducts. Our results can be interpreted as indicating that divicine autooxidizes both at extracellular level and into myoblasts thus inducing the release of free iron, which initiates oxidation of cellular proteins and lipids. DFO protects the cells by subtracting the free iron both at intracellular and extracellular level.

Original languageEnglish
JournalLife Sciences
Volume66
Issue number6
DOIs
Publication statusPublished - Dec 31 1999

Fingerprint

Myoblasts
Oxidants
Iron
Vicia faba
Deferoxamine
Oxidation
Proteins
Chelating Agents
L-Lactate Dehydrogenase
Culture Media
Lipids
divicine
Pharmaceutical Preparations
4-hydroxy-2-nonenal

Keywords

  • 4-hydroxynonenal adducts
  • Divicine
  • Free iron
  • Myoblasts
  • Oxidant stress
  • Protein carbonyls

ASJC Scopus subject areas

  • Pharmacology

Cite this

Ninfali, P., Perini, M. P., Bresolin, N., Aluigi, G., Cambiaggi, C., Ferrali, M., & Pompella, A. (1999). Iron release and oxidant damage in human myoblasts by divicine. Life Sciences, 66(6). https://doi.org/10.1016/S0024-3205(99)00625-6

Iron release and oxidant damage in human myoblasts by divicine. / Ninfali, Paolino; Perini, Maria Paola; Bresolin, Nereo; Aluigi, Gianfranca; Cambiaggi, Caterina; Ferrali, Marco; Pompella, Alfonso.

In: Life Sciences, Vol. 66, No. 6, 31.12.1999.

Research output: Contribution to journalArticle

Ninfali, P, Perini, MP, Bresolin, N, Aluigi, G, Cambiaggi, C, Ferrali, M & Pompella, A 1999, 'Iron release and oxidant damage in human myoblasts by divicine', Life Sciences, vol. 66, no. 6. https://doi.org/10.1016/S0024-3205(99)00625-6
Ninfali, Paolino ; Perini, Maria Paola ; Bresolin, Nereo ; Aluigi, Gianfranca ; Cambiaggi, Caterina ; Ferrali, Marco ; Pompella, Alfonso. / Iron release and oxidant damage in human myoblasts by divicine. In: Life Sciences. 1999 ; Vol. 66, No. 6.
@article{2b5f692bce2f4ebda5d1262b97b70c23,
title = "Iron release and oxidant damage in human myoblasts by divicine",
abstract = "Divicine is an aglycone derived from vicine, a glucosidic compound contained in fava beans (Vicia faba major or broad beans). In this study, we investigated the effect of divicine on cultured human myoblasts from normal subjects, in order to see if the drug may induce signs of oxidant stress in these cells. Myoblasts incubated 24 hours in the presence of 1 mM divicine, showed an increase of carbonyl groups and 4-hydroxynonenal (4-HNE) bound to cell proteins, as well as a significant release of iron and lactate dehydrogenase in the culture medium. Desferrioxamine (DFO), an iron chelator, significantly prevented protein oxidation and formation 4-HNE adducts. Our results can be interpreted as indicating that divicine autooxidizes both at extracellular level and into myoblasts thus inducing the release of free iron, which initiates oxidation of cellular proteins and lipids. DFO protects the cells by subtracting the free iron both at intracellular and extracellular level.",
keywords = "4-hydroxynonenal adducts, Divicine, Free iron, Myoblasts, Oxidant stress, Protein carbonyls",
author = "Paolino Ninfali and Perini, {Maria Paola} and Nereo Bresolin and Gianfranca Aluigi and Caterina Cambiaggi and Marco Ferrali and Alfonso Pompella",
year = "1999",
month = "12",
day = "31",
doi = "10.1016/S0024-3205(99)00625-6",
language = "English",
volume = "66",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Iron release and oxidant damage in human myoblasts by divicine

AU - Ninfali, Paolino

AU - Perini, Maria Paola

AU - Bresolin, Nereo

AU - Aluigi, Gianfranca

AU - Cambiaggi, Caterina

AU - Ferrali, Marco

AU - Pompella, Alfonso

PY - 1999/12/31

Y1 - 1999/12/31

N2 - Divicine is an aglycone derived from vicine, a glucosidic compound contained in fava beans (Vicia faba major or broad beans). In this study, we investigated the effect of divicine on cultured human myoblasts from normal subjects, in order to see if the drug may induce signs of oxidant stress in these cells. Myoblasts incubated 24 hours in the presence of 1 mM divicine, showed an increase of carbonyl groups and 4-hydroxynonenal (4-HNE) bound to cell proteins, as well as a significant release of iron and lactate dehydrogenase in the culture medium. Desferrioxamine (DFO), an iron chelator, significantly prevented protein oxidation and formation 4-HNE adducts. Our results can be interpreted as indicating that divicine autooxidizes both at extracellular level and into myoblasts thus inducing the release of free iron, which initiates oxidation of cellular proteins and lipids. DFO protects the cells by subtracting the free iron both at intracellular and extracellular level.

AB - Divicine is an aglycone derived from vicine, a glucosidic compound contained in fava beans (Vicia faba major or broad beans). In this study, we investigated the effect of divicine on cultured human myoblasts from normal subjects, in order to see if the drug may induce signs of oxidant stress in these cells. Myoblasts incubated 24 hours in the presence of 1 mM divicine, showed an increase of carbonyl groups and 4-hydroxynonenal (4-HNE) bound to cell proteins, as well as a significant release of iron and lactate dehydrogenase in the culture medium. Desferrioxamine (DFO), an iron chelator, significantly prevented protein oxidation and formation 4-HNE adducts. Our results can be interpreted as indicating that divicine autooxidizes both at extracellular level and into myoblasts thus inducing the release of free iron, which initiates oxidation of cellular proteins and lipids. DFO protects the cells by subtracting the free iron both at intracellular and extracellular level.

KW - 4-hydroxynonenal adducts

KW - Divicine

KW - Free iron

KW - Myoblasts

KW - Oxidant stress

KW - Protein carbonyls

UR - http://www.scopus.com/inward/record.url?scp=0003388041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0003388041&partnerID=8YFLogxK

U2 - 10.1016/S0024-3205(99)00625-6

DO - 10.1016/S0024-3205(99)00625-6

M3 - Article

C2 - 10794072

AN - SCOPUS:0003388041

VL - 66

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 6

ER -