Irradiation of Mesenchymal Stromal Cells With Low and High Doses of Alpha Particles Induces Senescence and/or Apoptosis

Nicola Alessio, Giuseppe Esposito, Giovanni Galano, Roberto De Rosa, Pasquale Anello, Gianfranco Peluso, Maria Antonella Tabocchini, Umberto Galderisi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The use of high-linear energy transfer charged particles is gaining attention as a medical tool because of the emission of radiations with an efficient cell-killing ability. Considerable interest has developed in the use of targeted alpha-particle therapy for the treatment of micrometastases. Moreover, the use of helium beams is gaining momentum, especially for treating pediatric tumors. We analyzed the effects of alpha particles on bone marrow mesenchymal stromal cells (MSCs), which have a subpopulation of stem cells capable of generating adipocytes, chondrocytes, and osteocytes. Further, these cells contribute toward maintenance of homeostasis in the body. MSCs were irradiated with low and high doses of alpha particles or X-rays and a comparative biological analysis was performed. At a low dose (40 mGy), alpha particles exhibited a limited negative effect on the biology of MSCs compared with X-rays. No significant perturbation of cell cycle was observed, and a minimal increase in apoptosis or senescence was detected. Self-renewal was preserved as revealed by the CFU assay. On the contrary, with 2000 mGy alpha particles, we observed adverse effects on the vitality, functionality, and stemness of MSCs. These results are the consequence of different proportion of cells targeted by alpha particles or X-rays and the quality of induced DNA damage. The present study suggests that radiotherapy with alpha particles may spare healthy stem cells more efficaciously than X-ray treatments, an observation that should be taken into consideration by physicians while planning irradiation of tumor areas close to stem cell niches, such as bone marrow. J. Cell. Biochem. 118: 2993–3002, 2017.

Original languageEnglish
Pages (from-to)2993-3002
Number of pages10
JournalJournal of Cellular Biochemistry
Volume118
Issue number9
DOIs
Publication statusPublished - Sep 1 2017

Fingerprint

Alpha Particles
Alpha particles
Mesenchymal Stromal Cells
Cells
Irradiation
Apoptosis
Stem cells
X-Rays
X rays
Tumors
Bone
Stem Cells
Stem Cell Niche
Linear Energy Transfer
Osteocytes
Neoplasm Micrometastasis
Helium
Pediatrics
Radiotherapy
Charged particles

Keywords

  • APOPTOSIS
  • CANCER THERAPY
  • IRRADIATION
  • MESENCHYMAL STROMAL CELLS
  • SENESCENCE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Irradiation of Mesenchymal Stromal Cells With Low and High Doses of Alpha Particles Induces Senescence and/or Apoptosis. / Alessio, Nicola; Esposito, Giuseppe; Galano, Giovanni; De Rosa, Roberto; Anello, Pasquale; Peluso, Gianfranco; Tabocchini, Maria Antonella; Galderisi, Umberto.

In: Journal of Cellular Biochemistry, Vol. 118, No. 9, 01.09.2017, p. 2993-3002.

Research output: Contribution to journalArticle

Alessio, Nicola ; Esposito, Giuseppe ; Galano, Giovanni ; De Rosa, Roberto ; Anello, Pasquale ; Peluso, Gianfranco ; Tabocchini, Maria Antonella ; Galderisi, Umberto. / Irradiation of Mesenchymal Stromal Cells With Low and High Doses of Alpha Particles Induces Senescence and/or Apoptosis. In: Journal of Cellular Biochemistry. 2017 ; Vol. 118, No. 9. pp. 2993-3002.
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