Is amyloid β-protein glycated in Alzheimer's disease?

Massimo Tabaton, George Perry, Mark Smith, Michael Vitek, Giovanna Angelini, Debora Dapino, Silvano Garibaldi, Damiano Zaccheo, Patrizio Odetti

Research output: Contribution to journalArticle

Abstract

Recent data suggest that protein glycation is involved in the process of amyloid formation in Alzheimer's disease (AD). To further investigate this issue, we analyzed the presence of advanced glycation end products (AGE) in soluble and insoluble forms of amyloid β-protein (βP) as well as in apolipoprotein E (apoE), a protein bound to amyloid deposits. Both proteins were extracted from cerebral cortex obtained from patients with AD and probed by immunoblotting with two antibodies specific for different AGE, already known to immunocytochemically label amyloid plaques. All the AGE antibodies failed to recognize either βP or apoE, whereas they reacted with synthetic βP glycated in vitro. These findings indicate that other proteins associated with amyloid deposits are candidates to be modified with AGE in Alzheimer's cerebral tissue.

Original languageEnglish
Pages (from-to)907-909
Number of pages3
JournalNeuroReport
Volume8
Issue number4
Publication statusPublished - 1997

Fingerprint

Amyloidogenic Proteins
Advanced Glycosylation End Products
Amyloid Plaques
Alzheimer Disease
Apolipoproteins E
Proteins
Antibodies
Immunoblotting
Amyloid
Cerebral Cortex

Keywords

  • Alzheimer's disease
  • Amyloid β-protein
  • Apolipoprotein E
  • Non-enzymatic glycation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Tabaton, M., Perry, G., Smith, M., Vitek, M., Angelini, G., Dapino, D., ... Odetti, P. (1997). Is amyloid β-protein glycated in Alzheimer's disease? NeuroReport, 8(4), 907-909.

Is amyloid β-protein glycated in Alzheimer's disease? / Tabaton, Massimo; Perry, George; Smith, Mark; Vitek, Michael; Angelini, Giovanna; Dapino, Debora; Garibaldi, Silvano; Zaccheo, Damiano; Odetti, Patrizio.

In: NeuroReport, Vol. 8, No. 4, 1997, p. 907-909.

Research output: Contribution to journalArticle

Tabaton, M, Perry, G, Smith, M, Vitek, M, Angelini, G, Dapino, D, Garibaldi, S, Zaccheo, D & Odetti, P 1997, 'Is amyloid β-protein glycated in Alzheimer's disease?', NeuroReport, vol. 8, no. 4, pp. 907-909.
Tabaton M, Perry G, Smith M, Vitek M, Angelini G, Dapino D et al. Is amyloid β-protein glycated in Alzheimer's disease? NeuroReport. 1997;8(4):907-909.
Tabaton, Massimo ; Perry, George ; Smith, Mark ; Vitek, Michael ; Angelini, Giovanna ; Dapino, Debora ; Garibaldi, Silvano ; Zaccheo, Damiano ; Odetti, Patrizio. / Is amyloid β-protein glycated in Alzheimer's disease?. In: NeuroReport. 1997 ; Vol. 8, No. 4. pp. 907-909.
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