E la lipoperossidazione responsabile del danno da riperfusione postischemica?

Translated title of the contribution: Is lipid peroxidation responsible for the damage caused by postischemic reperfusion?

A. Cargnoni, E. Pasini, C. Ceconi, R. Ferrari, S. Curello, M. Benigno, O. Visioli

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Peroxidation of membrane phospholipid polyunsaturated fatty acids is considered a major mechanism of the damage occurring during post-ischemic reperfusion. The evidences in support for this mechanism of damage are based on tissue malondialdehyde (MDA) quantitation by the thiobarbituric acid test (TBA-test). In an attempt to verify this topic we have subjected isolated and Langendorff perfused rabbit hearts to a period of 60 min of severe ischemia plus 30 min of reperfusion. At appropriate time points MDA was determined in the tissue by means of TBA-test and directly by reversed phase, high pressure, liquid chromatography (HPLC). We have found no correlation between the 2 compared assays. During reperfusion, there was the formation of non-lipid related, MDA like, TBA-reactive substance which leads to overestimation of the extent of lipid peroxidation. On the contrary, by direct HPLC quantitation, there was a decrease of tissue MDA during ischemia and during the early phases of reperfusion. Our results demonstrate that TBA-test is not a reliable index of lipid peroxidation in organ systems and that MDA accumulation does not precede the evidence of the functional alterations occurring on reperfusion of the previously ischemic myocardium. These results are of relevance in the understanding of the exact mechanism of reperfusion damage as, in the same experimental model, oxy radicals have been shown to be generated and antioxidants are protective.

Original languageItalian
Pages (from-to)123-128
Number of pages6
JournalCardiologia
Volume36
Issue number2
Publication statusPublished - Feb 1991

Fingerprint

Malondialdehyde
Lipid Peroxidation
Reperfusion
Ischemia
High Pressure Liquid Chromatography
Reperfusion Injury
Unsaturated Fatty Acids
Phospholipids
Myocardium
Theoretical Models
Antioxidants
Rabbits
Membranes
thiobarbituric acid

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Cargnoni, A., Pasini, E., Ceconi, C., Ferrari, R., Curello, S., Benigno, M., & Visioli, O. (1991). E la lipoperossidazione responsabile del danno da riperfusione postischemica? Cardiologia, 36(2), 123-128.

E la lipoperossidazione responsabile del danno da riperfusione postischemica? / Cargnoni, A.; Pasini, E.; Ceconi, C.; Ferrari, R.; Curello, S.; Benigno, M.; Visioli, O.

In: Cardiologia, Vol. 36, No. 2, 02.1991, p. 123-128.

Research output: Contribution to journalArticle

Cargnoni, A, Pasini, E, Ceconi, C, Ferrari, R, Curello, S, Benigno, M & Visioli, O 1991, 'E la lipoperossidazione responsabile del danno da riperfusione postischemica?', Cardiologia, vol. 36, no. 2, pp. 123-128.
Cargnoni A, Pasini E, Ceconi C, Ferrari R, Curello S, Benigno M et al. E la lipoperossidazione responsabile del danno da riperfusione postischemica? Cardiologia. 1991 Feb;36(2):123-128.
Cargnoni, A. ; Pasini, E. ; Ceconi, C. ; Ferrari, R. ; Curello, S. ; Benigno, M. ; Visioli, O. / E la lipoperossidazione responsabile del danno da riperfusione postischemica?. In: Cardiologia. 1991 ; Vol. 36, No. 2. pp. 123-128.
@article{96e0c5669d414e398c569bd3402db3e6,
title = "E la lipoperossidazione responsabile del danno da riperfusione postischemica?",
abstract = "Peroxidation of membrane phospholipid polyunsaturated fatty acids is considered a major mechanism of the damage occurring during post-ischemic reperfusion. The evidences in support for this mechanism of damage are based on tissue malondialdehyde (MDA) quantitation by the thiobarbituric acid test (TBA-test). In an attempt to verify this topic we have subjected isolated and Langendorff perfused rabbit hearts to a period of 60 min of severe ischemia plus 30 min of reperfusion. At appropriate time points MDA was determined in the tissue by means of TBA-test and directly by reversed phase, high pressure, liquid chromatography (HPLC). We have found no correlation between the 2 compared assays. During reperfusion, there was the formation of non-lipid related, MDA like, TBA-reactive substance which leads to overestimation of the extent of lipid peroxidation. On the contrary, by direct HPLC quantitation, there was a decrease of tissue MDA during ischemia and during the early phases of reperfusion. Our results demonstrate that TBA-test is not a reliable index of lipid peroxidation in organ systems and that MDA accumulation does not precede the evidence of the functional alterations occurring on reperfusion of the previously ischemic myocardium. These results are of relevance in the understanding of the exact mechanism of reperfusion damage as, in the same experimental model, oxy radicals have been shown to be generated and antioxidants are protective.",
author = "A. Cargnoni and E. Pasini and C. Ceconi and R. Ferrari and S. Curello and M. Benigno and O. Visioli",
year = "1991",
month = "2",
language = "Italian",
volume = "36",
pages = "123--128",
journal = "Cardiologia (Rome, Italy)",
issn = "0393-1978",
publisher = "Societa Italiana di Cardiologia",
number = "2",

}

TY - JOUR

T1 - E la lipoperossidazione responsabile del danno da riperfusione postischemica?

AU - Cargnoni, A.

AU - Pasini, E.

AU - Ceconi, C.

AU - Ferrari, R.

AU - Curello, S.

AU - Benigno, M.

AU - Visioli, O.

PY - 1991/2

Y1 - 1991/2

N2 - Peroxidation of membrane phospholipid polyunsaturated fatty acids is considered a major mechanism of the damage occurring during post-ischemic reperfusion. The evidences in support for this mechanism of damage are based on tissue malondialdehyde (MDA) quantitation by the thiobarbituric acid test (TBA-test). In an attempt to verify this topic we have subjected isolated and Langendorff perfused rabbit hearts to a period of 60 min of severe ischemia plus 30 min of reperfusion. At appropriate time points MDA was determined in the tissue by means of TBA-test and directly by reversed phase, high pressure, liquid chromatography (HPLC). We have found no correlation between the 2 compared assays. During reperfusion, there was the formation of non-lipid related, MDA like, TBA-reactive substance which leads to overestimation of the extent of lipid peroxidation. On the contrary, by direct HPLC quantitation, there was a decrease of tissue MDA during ischemia and during the early phases of reperfusion. Our results demonstrate that TBA-test is not a reliable index of lipid peroxidation in organ systems and that MDA accumulation does not precede the evidence of the functional alterations occurring on reperfusion of the previously ischemic myocardium. These results are of relevance in the understanding of the exact mechanism of reperfusion damage as, in the same experimental model, oxy radicals have been shown to be generated and antioxidants are protective.

AB - Peroxidation of membrane phospholipid polyunsaturated fatty acids is considered a major mechanism of the damage occurring during post-ischemic reperfusion. The evidences in support for this mechanism of damage are based on tissue malondialdehyde (MDA) quantitation by the thiobarbituric acid test (TBA-test). In an attempt to verify this topic we have subjected isolated and Langendorff perfused rabbit hearts to a period of 60 min of severe ischemia plus 30 min of reperfusion. At appropriate time points MDA was determined in the tissue by means of TBA-test and directly by reversed phase, high pressure, liquid chromatography (HPLC). We have found no correlation between the 2 compared assays. During reperfusion, there was the formation of non-lipid related, MDA like, TBA-reactive substance which leads to overestimation of the extent of lipid peroxidation. On the contrary, by direct HPLC quantitation, there was a decrease of tissue MDA during ischemia and during the early phases of reperfusion. Our results demonstrate that TBA-test is not a reliable index of lipid peroxidation in organ systems and that MDA accumulation does not precede the evidence of the functional alterations occurring on reperfusion of the previously ischemic myocardium. These results are of relevance in the understanding of the exact mechanism of reperfusion damage as, in the same experimental model, oxy radicals have been shown to be generated and antioxidants are protective.

UR - http://www.scopus.com/inward/record.url?scp=0026102863&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026102863&partnerID=8YFLogxK

M3 - Articolo

VL - 36

SP - 123

EP - 128

JO - Cardiologia (Rome, Italy)

JF - Cardiologia (Rome, Italy)

SN - 0393-1978

IS - 2

ER -