TY - JOUR
T1 - Is polyamine decrease a common feature of apoptosis? Evidence from γ rays- and heat shock-induced cell death
AU - Grassill, Emanuela
AU - Desiderio, Maria Alfonsina
AU - Bellesia, Enrica
AU - Salomoni, Paolo
AU - Benatti, Francesca
AU - Franceschi, Claudio
PY - 1995
Y1 - 1995
N2 - Here we report that in rat thymocytes undergoing apoptosis upon two different stimuli, such as heat shock treatment and γ irradiation, an early mRNA accumulation of ornithine decarboxylase (ODC) - the rate-limiting enzyme of polyamine biosynthesis was followed by a very marked increase in ODC activity (28-40 and 6-8-fold, respectively). However, polyamine levels started to decrease before the appearance of DNA laddering, being putrescine and spermidine strongly diminished (8-12 hs), and spermine even depleted (12 hs). Taken together with our previous data on another model of apoptosis, i.e., glucocorticoid-induced cell death, these results suggest that an imbalance of polyamine metabolism. i.e. a strong activation of ODC and a paradoxical decrease of the intracellular polyamine content, might be a general feature of the apoptotic process.
AB - Here we report that in rat thymocytes undergoing apoptosis upon two different stimuli, such as heat shock treatment and γ irradiation, an early mRNA accumulation of ornithine decarboxylase (ODC) - the rate-limiting enzyme of polyamine biosynthesis was followed by a very marked increase in ODC activity (28-40 and 6-8-fold, respectively). However, polyamine levels started to decrease before the appearance of DNA laddering, being putrescine and spermidine strongly diminished (8-12 hs), and spermine even depleted (12 hs). Taken together with our previous data on another model of apoptosis, i.e., glucocorticoid-induced cell death, these results suggest that an imbalance of polyamine metabolism. i.e. a strong activation of ODC and a paradoxical decrease of the intracellular polyamine content, might be a general feature of the apoptotic process.
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U2 - 10.1006/bbrc.1995.2679
DO - 10.1006/bbrc.1995.2679
M3 - Article
C2 - 7488168
AN - SCOPUS:0028871736
VL - 216
SP - 708
EP - 714
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -