Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples?

Enrico Tortoli, Pasquale Urbano, Fiorella Marcelli, Tullia M. Simonetti, Daniela M. Cirilloa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cobas Amplicor MTB and later Cobas TaqMan MTB were used to test a very large series of consecutive specimens received for tuberculosis diagnosis. Performance parameters were estimated and compared overall and for separate specimen categories. Both systems showed excellent specificity, and that of TaqMan was the higher. The sensitivities were similar but satisfactory only with respiratory specimens and smear-positive samples.

Original languageEnglish
Pages (from-to)2810-2813
Number of pages4
JournalJournal of Clinical Microbiology
Volume50
Issue number8
DOIs
Publication statusPublished - Aug 2012

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Mycobacterium tuberculosis
Real-Time Polymerase Chain Reaction
Tuberculosis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)

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Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples? / Tortoli, Enrico; Urbano, Pasquale; Marcelli, Fiorella; Simonetti, Tullia M.; Cirilloa, Daniela M.

In: Journal of Clinical Microbiology, Vol. 50, No. 8, 08.2012, p. 2810-2813.

Research output: Contribution to journalArticle

Tortoli, Enrico ; Urbano, Pasquale ; Marcelli, Fiorella ; Simonetti, Tullia M. ; Cirilloa, Daniela M. / Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples?. In: Journal of Clinical Microbiology. 2012 ; Vol. 50, No. 8. pp. 2810-2813.
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