Isoelectric focusing and electrophoresis at subzero temperatures

Michele Perrella, Adriana Heyda, Andrea Mosca, Luigi Rossi-Bernardi

Research output: Contribution to journalArticlepeer-review


Electrophoretic and isoelectric focusing separations have been achieved at -20 to -30°C, i.e., at temperatures considerably lower than previously reported by using as supporting media gels of acrylamide-methylacrylate copolymers and dimenthylsulfoxide-water mixtures. Hybrids of human and sickle cell hemoglobin and partially oxidized human carboxyhemoglobin have been separated in the temperature range -20 to -30°C, both by a discontinuous buffer gel electrophoresis and by isoelectric focusing.

Original languageEnglish
Pages (from-to)212-224
Number of pages13
JournalAnalytical Biochemistry
Issue number1
Publication statusPublished - Jul 15 1978

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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