Isoelectric focusing and electrophoresis at subzero temperatures

Michele Perrella, Adriana Heyda, Andrea Mosca, Luigi Rossi-Bernardi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Electrophoretic and isoelectric focusing separations have been achieved at -20 to -30°C, i.e., at temperatures considerably lower than previously reported by using as supporting media gels of acrylamide-methylacrylate copolymers and dimenthylsulfoxide-water mixtures. Hybrids of human and sickle cell hemoglobin and partially oxidized human carboxyhemoglobin have been separated in the temperature range -20 to -30°C, both by a discontinuous buffer gel electrophoresis and by isoelectric focusing.

Original languageEnglish
Pages (from-to)212-224
Number of pages13
JournalAnalytical Biochemistry
Volume88
Issue number1
DOIs
Publication statusPublished - Jul 15 1978

Fingerprint

Isoelectric Focusing
Electrophoresis
Gels
Carboxyhemoglobin
Sickle Hemoglobin
Temperature
Acrylamide
Buffers
Hemoglobins
Copolymers
Water

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isoelectric focusing and electrophoresis at subzero temperatures. / Perrella, Michele; Heyda, Adriana; Mosca, Andrea; Rossi-Bernardi, Luigi.

In: Analytical Biochemistry, Vol. 88, No. 1, 15.07.1978, p. 212-224.

Research output: Contribution to journalArticle

Perrella, Michele ; Heyda, Adriana ; Mosca, Andrea ; Rossi-Bernardi, Luigi. / Isoelectric focusing and electrophoresis at subzero temperatures. In: Analytical Biochemistry. 1978 ; Vol. 88, No. 1. pp. 212-224.
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