Isoelectric focusing herpes simplex virus-gB overlay study in brainstem encephalitis

G. V. Martino, L. M E Grimaldi, D. M. Franciotta, G. Piccolo, R. Brustia, L. Minoli, G. V. Melzi d'Eril

Research output: Contribution to journalArticlepeer-review


We report a case of herpetic brainstem encephalitis (HBE) retrospectively diagnosed in an adult patient. Conventional immunovirological studies failed to disclose the etiology of this patient's affection. An isoelectric focusing-antigen overlay (IEF-O) technique showed that the target of one of the four cerebrospinal fluid oligoclonal bands was herpes simplex virus (HSV)-1 glycoprotein B, indicating a specific anti-HSV immunoresponse restricted to the CNS. IEF-O may represent a useful support for in vivo diagnosis of HBE.

Original languageEnglish
Pages (from-to)364-367
Number of pages4
JournalActa Neurologica Scandinavica
Issue number6
Publication statusPublished - 1990


  • brainstem encephalitis
  • herpes simplex virus type 1
  • isoelectric focusing antigen overlay
  • oligoclonal bands

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)


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