Abstract
Background: Early diagnosis of perinatally acquired HIV-infection is based on either direct HIV detection - by means of viral culture and/or PCR - or anti-HIV antibody detection. However, due to the passive, transplacental passage of maternal immunoglobulin G, antibody detection is nor reliable until 15-18 months of age. In this regard, clonotypic analysis of specific antibodies performed by isoelectricfocusing and reverse blotting (IEF-RB) can be very helpful, as it recognizes possibly different patterns between mother and infant. Objectives: We used IEF-RB in order to analyze the kinetics of development of anti-HIV antibodies in infants born to seropositive mothers. Study design: Sera from ten mother/infant pairs (all mothers were HIV-infected) were retrospectively analyzed in order to detect different patterns, between mother and infant, in anti-gp120 V3-loop clonotype. Results: We diagnosed the real HIV status of the examined infants no later than month 6 and in one case as early as month 2. Conclusions: Considering the small size of sample number, these data are preliminary and should be confirmed by larger scale studies. However, they show IEF-RB, when applied to infants born to seropositive mothers, may be useful in evaluating the infants' dynamics of anti-HIV humoral immune response.
| Original language | English |
|---|---|
| Pages (from-to) | 203-210 |
| Number of pages | 8 |
| Journal | Journal of Clinical Virology |
| Volume | 11 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Dec 1998 |
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Keywords
- HIV-antibodies
- HIV-serodiagnosis
- Isoelectricfocusing
- Perinatal infection
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Virology
- Immunology and Allergy
- Infectious Diseases
Cite this
Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection. / Del Bono, Valerio; Biselli, Roberto; Nisini, Roberto; Loomis-Price, Lawrence D.; Loy, Anna; Lorusso, Carolina; Bassetti, Dante; D'Amelio, Raffaele.
In: Journal of Clinical Virology, Vol. 11, No. 3, 12.1998, p. 203-210.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection
AU - Del Bono, Valerio
AU - Biselli, Roberto
AU - Nisini, Roberto
AU - Loomis-Price, Lawrence D.
AU - Loy, Anna
AU - Lorusso, Carolina
AU - Bassetti, Dante
AU - D'Amelio, Raffaele
PY - 1998/12
Y1 - 1998/12
N2 - Background: Early diagnosis of perinatally acquired HIV-infection is based on either direct HIV detection - by means of viral culture and/or PCR - or anti-HIV antibody detection. However, due to the passive, transplacental passage of maternal immunoglobulin G, antibody detection is nor reliable until 15-18 months of age. In this regard, clonotypic analysis of specific antibodies performed by isoelectricfocusing and reverse blotting (IEF-RB) can be very helpful, as it recognizes possibly different patterns between mother and infant. Objectives: We used IEF-RB in order to analyze the kinetics of development of anti-HIV antibodies in infants born to seropositive mothers. Study design: Sera from ten mother/infant pairs (all mothers were HIV-infected) were retrospectively analyzed in order to detect different patterns, between mother and infant, in anti-gp120 V3-loop clonotype. Results: We diagnosed the real HIV status of the examined infants no later than month 6 and in one case as early as month 2. Conclusions: Considering the small size of sample number, these data are preliminary and should be confirmed by larger scale studies. However, they show IEF-RB, when applied to infants born to seropositive mothers, may be useful in evaluating the infants' dynamics of anti-HIV humoral immune response.
AB - Background: Early diagnosis of perinatally acquired HIV-infection is based on either direct HIV detection - by means of viral culture and/or PCR - or anti-HIV antibody detection. However, due to the passive, transplacental passage of maternal immunoglobulin G, antibody detection is nor reliable until 15-18 months of age. In this regard, clonotypic analysis of specific antibodies performed by isoelectricfocusing and reverse blotting (IEF-RB) can be very helpful, as it recognizes possibly different patterns between mother and infant. Objectives: We used IEF-RB in order to analyze the kinetics of development of anti-HIV antibodies in infants born to seropositive mothers. Study design: Sera from ten mother/infant pairs (all mothers were HIV-infected) were retrospectively analyzed in order to detect different patterns, between mother and infant, in anti-gp120 V3-loop clonotype. Results: We diagnosed the real HIV status of the examined infants no later than month 6 and in one case as early as month 2. Conclusions: Considering the small size of sample number, these data are preliminary and should be confirmed by larger scale studies. However, they show IEF-RB, when applied to infants born to seropositive mothers, may be useful in evaluating the infants' dynamics of anti-HIV humoral immune response.
KW - HIV-antibodies
KW - HIV-serodiagnosis
KW - Isoelectricfocusing
KW - Perinatal infection
UR - http://www.scopus.com/inward/record.url?scp=0032433798&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032433798&partnerID=8YFLogxK
U2 - 10.1016/S0928-0197(98)00050-6
DO - 10.1016/S0928-0197(98)00050-6
M3 - Article
C2 - 9949956
AN - SCOPUS:0032433798
VL - 11
SP - 203
EP - 210
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
IS - 3
ER -