Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection

Valerio Del Bono, Roberto Biselli, Roberto Nisini, Lawrence D. Loomis-Price, Anna Loy, Carolina Lorusso, Dante Bassetti, Raffaele D'Amelio

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Early diagnosis of perinatally acquired HIV-infection is based on either direct HIV detection - by means of viral culture and/or PCR - or anti-HIV antibody detection. However, due to the passive, transplacental passage of maternal immunoglobulin G, antibody detection is nor reliable until 15-18 months of age. In this regard, clonotypic analysis of specific antibodies performed by isoelectricfocusing and reverse blotting (IEF-RB) can be very helpful, as it recognizes possibly different patterns between mother and infant. Objectives: We used IEF-RB in order to analyze the kinetics of development of anti-HIV antibodies in infants born to seropositive mothers. Study design: Sera from ten mother/infant pairs (all mothers were HIV-infected) were retrospectively analyzed in order to detect different patterns, between mother and infant, in anti-gp120 V3-loop clonotype. Results: We diagnosed the real HIV status of the examined infants no later than month 6 and in one case as early as month 2. Conclusions: Considering the small size of sample number, these data are preliminary and should be confirmed by larger scale studies. However, they show IEF-RB, when applied to infants born to seropositive mothers, may be useful in evaluating the infants' dynamics of anti-HIV humoral immune response.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalJournal of Clinical Virology
Volume11
Issue number3
DOIs
Publication statusPublished - Dec 1998

Fingerprint

HIV Infections
Mothers
Pediatrics
HIV
HIV Antibodies
Anti-Idiotypic Antibodies
Antibodies
Humoral Immunity
Sample Size
Early Diagnosis
Immunoglobulin G
Polymerase Chain Reaction
Serum

Keywords

  • HIV-antibodies
  • HIV-serodiagnosis
  • Isoelectricfocusing
  • Perinatal infection

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Del Bono, V., Biselli, R., Nisini, R., Loomis-Price, L. D., Loy, A., Lorusso, C., ... D'Amelio, R. (1998). Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection. Journal of Clinical Virology, 11(3), 203-210. https://doi.org/10.1016/S0928-0197(98)00050-6

Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection. / Del Bono, Valerio; Biselli, Roberto; Nisini, Roberto; Loomis-Price, Lawrence D.; Loy, Anna; Lorusso, Carolina; Bassetti, Dante; D'Amelio, Raffaele.

In: Journal of Clinical Virology, Vol. 11, No. 3, 12.1998, p. 203-210.

Research output: Contribution to journalArticle

Del Bono, V, Biselli, R, Nisini, R, Loomis-Price, LD, Loy, A, Lorusso, C, Bassetti, D & D'Amelio, R 1998, 'Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection', Journal of Clinical Virology, vol. 11, no. 3, pp. 203-210. https://doi.org/10.1016/S0928-0197(98)00050-6
Del Bono, Valerio ; Biselli, Roberto ; Nisini, Roberto ; Loomis-Price, Lawrence D. ; Loy, Anna ; Lorusso, Carolina ; Bassetti, Dante ; D'Amelio, Raffaele. / Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection. In: Journal of Clinical Virology. 1998 ; Vol. 11, No. 3. pp. 203-210.
@article{116892df1a7b4bf3aa2fd3e22c726ed8,
title = "Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection",
abstract = "Background: Early diagnosis of perinatally acquired HIV-infection is based on either direct HIV detection - by means of viral culture and/or PCR - or anti-HIV antibody detection. However, due to the passive, transplacental passage of maternal immunoglobulin G, antibody detection is nor reliable until 15-18 months of age. In this regard, clonotypic analysis of specific antibodies performed by isoelectricfocusing and reverse blotting (IEF-RB) can be very helpful, as it recognizes possibly different patterns between mother and infant. Objectives: We used IEF-RB in order to analyze the kinetics of development of anti-HIV antibodies in infants born to seropositive mothers. Study design: Sera from ten mother/infant pairs (all mothers were HIV-infected) were retrospectively analyzed in order to detect different patterns, between mother and infant, in anti-gp120 V3-loop clonotype. Results: We diagnosed the real HIV status of the examined infants no later than month 6 and in one case as early as month 2. Conclusions: Considering the small size of sample number, these data are preliminary and should be confirmed by larger scale studies. However, they show IEF-RB, when applied to infants born to seropositive mothers, may be useful in evaluating the infants' dynamics of anti-HIV humoral immune response.",
keywords = "HIV-antibodies, HIV-serodiagnosis, Isoelectricfocusing, Perinatal infection",
author = "{Del Bono}, Valerio and Roberto Biselli and Roberto Nisini and Loomis-Price, {Lawrence D.} and Anna Loy and Carolina Lorusso and Dante Bassetti and Raffaele D'Amelio",
year = "1998",
month = "12",
doi = "10.1016/S0928-0197(98)00050-6",
language = "English",
volume = "11",
pages = "203--210",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Isoelectricfocusing and reverse blotting as a diagnostic tool in pediatric HIV infection

AU - Del Bono, Valerio

AU - Biselli, Roberto

AU - Nisini, Roberto

AU - Loomis-Price, Lawrence D.

AU - Loy, Anna

AU - Lorusso, Carolina

AU - Bassetti, Dante

AU - D'Amelio, Raffaele

PY - 1998/12

Y1 - 1998/12

N2 - Background: Early diagnosis of perinatally acquired HIV-infection is based on either direct HIV detection - by means of viral culture and/or PCR - or anti-HIV antibody detection. However, due to the passive, transplacental passage of maternal immunoglobulin G, antibody detection is nor reliable until 15-18 months of age. In this regard, clonotypic analysis of specific antibodies performed by isoelectricfocusing and reverse blotting (IEF-RB) can be very helpful, as it recognizes possibly different patterns between mother and infant. Objectives: We used IEF-RB in order to analyze the kinetics of development of anti-HIV antibodies in infants born to seropositive mothers. Study design: Sera from ten mother/infant pairs (all mothers were HIV-infected) were retrospectively analyzed in order to detect different patterns, between mother and infant, in anti-gp120 V3-loop clonotype. Results: We diagnosed the real HIV status of the examined infants no later than month 6 and in one case as early as month 2. Conclusions: Considering the small size of sample number, these data are preliminary and should be confirmed by larger scale studies. However, they show IEF-RB, when applied to infants born to seropositive mothers, may be useful in evaluating the infants' dynamics of anti-HIV humoral immune response.

AB - Background: Early diagnosis of perinatally acquired HIV-infection is based on either direct HIV detection - by means of viral culture and/or PCR - or anti-HIV antibody detection. However, due to the passive, transplacental passage of maternal immunoglobulin G, antibody detection is nor reliable until 15-18 months of age. In this regard, clonotypic analysis of specific antibodies performed by isoelectricfocusing and reverse blotting (IEF-RB) can be very helpful, as it recognizes possibly different patterns between mother and infant. Objectives: We used IEF-RB in order to analyze the kinetics of development of anti-HIV antibodies in infants born to seropositive mothers. Study design: Sera from ten mother/infant pairs (all mothers were HIV-infected) were retrospectively analyzed in order to detect different patterns, between mother and infant, in anti-gp120 V3-loop clonotype. Results: We diagnosed the real HIV status of the examined infants no later than month 6 and in one case as early as month 2. Conclusions: Considering the small size of sample number, these data are preliminary and should be confirmed by larger scale studies. However, they show IEF-RB, when applied to infants born to seropositive mothers, may be useful in evaluating the infants' dynamics of anti-HIV humoral immune response.

KW - HIV-antibodies

KW - HIV-serodiagnosis

KW - Isoelectricfocusing

KW - Perinatal infection

UR - http://www.scopus.com/inward/record.url?scp=0032433798&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032433798&partnerID=8YFLogxK

U2 - 10.1016/S0928-0197(98)00050-6

DO - 10.1016/S0928-0197(98)00050-6

M3 - Article

VL - 11

SP - 203

EP - 210

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

IS - 3

ER -