Isoenzyme analysis of Entamoeba histolytica for evaluation of pathogenicity: detection of zymodeme XIX in South America.

G. Di Perri, M. Strosselli, A. Parisi, A. Malfitano, G. Carnevale, L. Minoli

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Abstract

Authors report the results of the isoenzyme analysis of strains of Entamoeba histolytica isolated from international travellers. This recently developed method allows the detection of pathogenic strains by evaluating the electrophoretic mobility of cytoplasmic enzymes and was proved to be more reliable and quickly feasible than previous ones. The experience reported refers to three strains isolated from travellers coming from Ecuador, Brazil and Indonesia, respectively; the zymodeme XIX (in accordance with the Sargeaunt's classification) was identified in all the three cases. This zymodeme has been first detected in 1981 and should currently be considered rare; moreover, it has never been previously reported from the Americas.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalLa Ricerca in Clinica e in Laboratorio
Volume19
Issue number2
Publication statusPublished - Apr 1989

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ASJC Scopus subject areas

  • Clinical Biochemistry

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Di Perri, G., Strosselli, M., Parisi, A., Malfitano, A., Carnevale, G., & Minoli, L. (1989). Isoenzyme analysis of Entamoeba histolytica for evaluation of pathogenicity: detection of zymodeme XIX in South America. La Ricerca in Clinica e in Laboratorio, 19(2), 179-185.