Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency

B. Iovine, M. Garofalo, M. Orefice, V. Giannini, F. Gasparri, G. Monfrecola, M. A. Bevilacqua

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Abstract

The isoflavones daidzein and genistein are natural compounds which have anti-inflammatory and photoprotective activities, and may be effective in the repair of ultraviolet (UV)-induced photodamage. In this study, an alcoholic solution of aglycone isoflavones with a genistein:daidzein ratio of 1:4 [Rottapharm (RPH)-aglycone] was examined for its effects on the repair of DNA damage induced by a single dose of UVB irradiation (20 mJ/cm2). For this purpose, human skin cells were first UVB-irradiated and then treated with RPH-aglycone. Comet assay analysis was used to estimate the UVB-induced DNA damage at different time points after treatment by measuring the tail moment parameter. We found that treatment with 10 μmol/L RPH-aglycone solution resulted in a significantly reduced tail moment at 1 h after treatment, and 34-35% enhancement of damage repair at 4 h after treatment. These results suggest that isoflavone aglycones are protective against UVB-induced DNA damage.

Original languageEnglish
Pages (from-to)391-394
Number of pages4
JournalClinical and Experimental Dermatology
Volume39
Issue number3
DOIs
Publication statusPublished - 2014

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ASJC Scopus subject areas

  • Dermatology
  • Medicine(all)

Cite this

Iovine, B., Garofalo, M., Orefice, M., Giannini, V., Gasparri, F., Monfrecola, G., & Bevilacqua, M. A. (2014). Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency. Clinical and Experimental Dermatology, 39(3), 391-394. https://doi.org/10.1111/ced.12290