Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency

B. Iovine; M. Garofalo; M. Orefice; V. Giannini; F. Gasparri; G. Monfrecola; M. A. Bevilacqua

doi:10.1111/ced.12290

Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency

B. Iovine, M. Garofalo, M. Orefice, V. Giannini, F. Gasparri, G. Monfrecola, M. A. Bevilacqua

Istituto Oncologico Candiolo

Research output: Contribution to journal › Article › peer-review

Abstract

The isoflavones daidzein and genistein are natural compounds which have anti-inflammatory and photoprotective activities, and may be effective in the repair of ultraviolet (UV)-induced photodamage. In this study, an alcoholic solution of aglycone isoflavones with a genistein:daidzein ratio of 1:4 [Rottapharm (RPH)-aglycone] was examined for its effects on the repair of DNA damage induced by a single dose of UVB irradiation (20 mJ/cm²). For this purpose, human skin cells were first UVB-irradiated and then treated with RPH-aglycone. Comet assay analysis was used to estimate the UVB-induced DNA damage at different time points after treatment by measuring the tail moment parameter. We found that treatment with 10 μmol/L RPH-aglycone solution resulted in a significantly reduced tail moment at 1 h after treatment, and 34-35% enhancement of damage repair at 4 h after treatment. These results suggest that isoflavone aglycones are protective against UVB-induced DNA damage.

Original language	English
Pages (from-to)	391-394
Number of pages	4
Journal	Clinical and Experimental Dermatology
Volume	39
Issue number	3
DOIs	https://doi.org/10.1111/ced.12290
Publication status	Published - 2014

ASJC Scopus subject areas

Dermatology
Medicine(all)

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title = "Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency",

abstract = "The isoflavones daidzein and genistein are natural compounds which have anti-inflammatory and photoprotective activities, and may be effective in the repair of ultraviolet (UV)-induced photodamage. In this study, an alcoholic solution of aglycone isoflavones with a genistein:daidzein ratio of 1:4 [Rottapharm (RPH)-aglycone] was examined for its effects on the repair of DNA damage induced by a single dose of UVB irradiation (20 mJ/cm2). For this purpose, human skin cells were first UVB-irradiated and then treated with RPH-aglycone. Comet assay analysis was used to estimate the UVB-induced DNA damage at different time points after treatment by measuring the tail moment parameter. We found that treatment with 10 μmol/L RPH-aglycone solution resulted in a significantly reduced tail moment at 1 h after treatment, and 34-35% enhancement of damage repair at 4 h after treatment. These results suggest that isoflavone aglycones are protective against UVB-induced DNA damage.",

author = "B. Iovine and M. Garofalo and M. Orefice and V. Giannini and F. Gasparri and G. Monfrecola and Bevilacqua, {M. A.}",

year = "2014",

doi = "10.1111/ced.12290",

language = "English",

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AU - Iovine, B.

AU - Garofalo, M.

AU - Orefice, M.

AU - Giannini, V.

AU - Gasparri, F.

AU - Monfrecola, G.

AU - Bevilacqua, M. A.

PY - 2014

Y1 - 2014

N2 - The isoflavones daidzein and genistein are natural compounds which have anti-inflammatory and photoprotective activities, and may be effective in the repair of ultraviolet (UV)-induced photodamage. In this study, an alcoholic solution of aglycone isoflavones with a genistein:daidzein ratio of 1:4 [Rottapharm (RPH)-aglycone] was examined for its effects on the repair of DNA damage induced by a single dose of UVB irradiation (20 mJ/cm2). For this purpose, human skin cells were first UVB-irradiated and then treated with RPH-aglycone. Comet assay analysis was used to estimate the UVB-induced DNA damage at different time points after treatment by measuring the tail moment parameter. We found that treatment with 10 μmol/L RPH-aglycone solution resulted in a significantly reduced tail moment at 1 h after treatment, and 34-35% enhancement of damage repair at 4 h after treatment. These results suggest that isoflavone aglycones are protective against UVB-induced DNA damage.

AB - The isoflavones daidzein and genistein are natural compounds which have anti-inflammatory and photoprotective activities, and may be effective in the repair of ultraviolet (UV)-induced photodamage. In this study, an alcoholic solution of aglycone isoflavones with a genistein:daidzein ratio of 1:4 [Rottapharm (RPH)-aglycone] was examined for its effects on the repair of DNA damage induced by a single dose of UVB irradiation (20 mJ/cm2). For this purpose, human skin cells were first UVB-irradiated and then treated with RPH-aglycone. Comet assay analysis was used to estimate the UVB-induced DNA damage at different time points after treatment by measuring the tail moment parameter. We found that treatment with 10 μmol/L RPH-aglycone solution resulted in a significantly reduced tail moment at 1 h after treatment, and 34-35% enhancement of damage repair at 4 h after treatment. These results suggest that isoflavone aglycones are protective against UVB-induced DNA damage.

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Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency

Abstract

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