Abstract
The isoflavones daidzein and genistein are natural compounds which have anti-inflammatory and photoprotective activities, and may be effective in the repair of ultraviolet (UV)-induced photodamage. In this study, an alcoholic solution of aglycone isoflavones with a genistein:daidzein ratio of 1:4 [Rottapharm (RPH)-aglycone] was examined for its effects on the repair of DNA damage induced by a single dose of UVB irradiation (20 mJ/cm2). For this purpose, human skin cells were first UVB-irradiated and then treated with RPH-aglycone. Comet assay analysis was used to estimate the UVB-induced DNA damage at different time points after treatment by measuring the tail moment parameter. We found that treatment with 10 μmol/L RPH-aglycone solution resulted in a significantly reduced tail moment at 1 h after treatment, and 34-35% enhancement of damage repair at 4 h after treatment. These results suggest that isoflavone aglycones are protective against UVB-induced DNA damage.
| Original language | English |
|---|---|
| Pages (from-to) | 391-394 |
| Number of pages | 4 |
| Journal | Clinical and Experimental Dermatology |
| Volume | 39 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 |
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ASJC Scopus subject areas
- Dermatology
- Medicine(all)
Cite this
Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency. / Iovine, B.; Garofalo, M.; Orefice, M.; Giannini, V.; Gasparri, F.; Monfrecola, G.; Bevilacqua, M. A.
In: Clinical and Experimental Dermatology, Vol. 39, No. 3, 2014, p. 391-394.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency
AU - Iovine, B.
AU - Garofalo, M.
AU - Orefice, M.
AU - Giannini, V.
AU - Gasparri, F.
AU - Monfrecola, G.
AU - Bevilacqua, M. A.
PY - 2014
Y1 - 2014
N2 - The isoflavones daidzein and genistein are natural compounds which have anti-inflammatory and photoprotective activities, and may be effective in the repair of ultraviolet (UV)-induced photodamage. In this study, an alcoholic solution of aglycone isoflavones with a genistein:daidzein ratio of 1:4 [Rottapharm (RPH)-aglycone] was examined for its effects on the repair of DNA damage induced by a single dose of UVB irradiation (20 mJ/cm2). For this purpose, human skin cells were first UVB-irradiated and then treated with RPH-aglycone. Comet assay analysis was used to estimate the UVB-induced DNA damage at different time points after treatment by measuring the tail moment parameter. We found that treatment with 10 μmol/L RPH-aglycone solution resulted in a significantly reduced tail moment at 1 h after treatment, and 34-35% enhancement of damage repair at 4 h after treatment. These results suggest that isoflavone aglycones are protective against UVB-induced DNA damage.
AB - The isoflavones daidzein and genistein are natural compounds which have anti-inflammatory and photoprotective activities, and may be effective in the repair of ultraviolet (UV)-induced photodamage. In this study, an alcoholic solution of aglycone isoflavones with a genistein:daidzein ratio of 1:4 [Rottapharm (RPH)-aglycone] was examined for its effects on the repair of DNA damage induced by a single dose of UVB irradiation (20 mJ/cm2). For this purpose, human skin cells were first UVB-irradiated and then treated with RPH-aglycone. Comet assay analysis was used to estimate the UVB-induced DNA damage at different time points after treatment by measuring the tail moment parameter. We found that treatment with 10 μmol/L RPH-aglycone solution resulted in a significantly reduced tail moment at 1 h after treatment, and 34-35% enhancement of damage repair at 4 h after treatment. These results suggest that isoflavone aglycones are protective against UVB-induced DNA damage.
UR - http://www.scopus.com/inward/record.url?scp=84898943031&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898943031&partnerID=8YFLogxK
U2 - 10.1111/ced.12290
DO - 10.1111/ced.12290
M3 - Article
C2 - 24635083
AN - SCOPUS:84898943031
VL - 39
SP - 391
EP - 394
JO - Clinical and Experimental Dermatology
JF - Clinical and Experimental Dermatology
SN - 0307-6938
IS - 3
ER -