Isolate and culture precursor cells from the adult periventricular area

Chiara Cavazzin, Margherita Neri, Angela Gritti

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Due to the complexity of the NSC niche organization, the lack of specific NSC markers and the difficulty of long-term tracking these cells and their progeny in vivo the functional properties of the endogenous NSCs remain largely unexplored. These limitations have led to the development of methodologies to efficiently isolate, expand, and differentiate NSCs ex vivo. We describe here the peculiarities of the neurosphere assay (NSA) as a methodology that allows to efficiently isolate, expand, and differentiate somatic NSCs derived from the adult forebrain periventricular region while preserving proliferation, self-renewal, and multipotency, the main attributes that provide their functional identification.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages25-40
Number of pages16
Volume1059
ISBN (Print)9781627035736
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1059
ISSN (Print)10643745

Keywords

  • Brain
  • Cell cultures
  • Cell proliferation
  • Multipotency
  • Murine models
  • Neural stem cells
  • Self-renewal
  • Subventricular zone

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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